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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human NADH-cytochrome b5 reductase-like (CYB5RL) Standard Curve.)

Human NADH-Cytochrome b5 Reductase-Like (CYB5RL) ELISA Kit | CYB5RL elisa kit

Human NADH-Cytochrome b5 Reductase-Like (CYB5RL) ELISA Kit

Reactivity
Human
Synonyms
NADH-Cytochrome b5 Reductase-Like (CYB5RL); Human NADH-Cytochrome b5 Reductase-Like (CYB5RL) ELISA Kit; CYB5RL; RP11-446E24.1; CYB5RL elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human NADH-cytochrome b5 reductase-like (CYB5RL) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYB5RL. No significant cross-reactivity or interference between Human CYB5RL and analogues was observed.
Sequence Length
315
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human NADH-cytochrome b5 reductase-like (CYB5RL) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human NADH-cytochrome b5 reductase-like (CYB5RL) Standard Curve.)
Related Product Information for CYB5RL elisa kit
Description: This Human NADH-cytochrome b5 reductase-like (CYB5RL) ELISA Kit employs a two-site sandwich ELISA to quantitate CYB5RL in samples. An antibody specific for CYB5RL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYB5RL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5RL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5RL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: CYB5RL (Cytochrome B5 Reductase Like) is a Protein Coding gene. Among its related pathways are Metabolism and Amino sugar and nucleotide sugar metabolism. GO annotations related to CYB5RL include oxidoreductase activity and cytochrome-b5 reductase activity, acting on NAD(P)H. An important paralog of CYB5RL is ENSG00000256407. NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
NADH-cytochrome b5 reductase-like isoform 1
NCBI Official Synonym Full Names
cytochrome b5 reductase like
NCBI Official Symbol
CYB5RL
NCBI Protein Information
NADH-cytochrome b5 reductase-like
UniProt Protein Name
NADH-cytochrome b5 reductase-like
UniProt Gene Name
CYB5RL
UniProt Entry Name
NB5R5_HUMAN

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Product Notes

The Human CYB5RL cyb5rl (Catalog #AAA9715847) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715847 ELISA Kit recognizes Human CYB5RL. It is sometimes possible for the material contained within the vial of "NADH-Cytochrome b5 Reductase-Like (CYB5RL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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