Rabbit MBD2 Polyclonal Antibody | anti-MBD2 antibody

MBD2 Polyclonal Antibody

Cat. Num. AAA2555772

• Gene Names: MBD2; DMTase; NY-CO-41
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: MBD2; Polyclonal Antibody; MBD2 Polyclonal Antibody; Demethylase; DMTase; MBD 2; Mbd2; MBD2a; Methyl CpG binding domain protein 2; Methyl CpG binding protein MBD2; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY CO 41; anti-MBD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 302

• Applicable Applications for anti-MBD2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:200-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human MBD2
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MBD2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat lung using MBD2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using MBD2 antibody.)

Related Product Information for anti-MBD2 antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-MBD2 antibody

Cancer; Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4505117
• NCBI GeneID: 8932
• NCBI Accession #: NP_003918.1
• NCBI GenBank Nucleotide #: NM_003927.5
• UniProt Accession #: Q9UBB5; O95242; Q9UIS8
• Molecular Weight: Calculated: 31kDa; 43kDa; Observed: 53kDa
• NCBI Official Full Name: methyl-CpG-binding domain protein 2 isoform 1
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 2
• NCBI Official Symbol: MBD2
• NCBI Official Synonym Symbols: DMTase; NY-CO-41
• NCBI Protein Information: methyl-CpG-binding domain protein 2
• UniProt Protein Name: Methyl-CpG-binding domain protein 2
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD2
• UniProt Synonym Gene Names: DMTase

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.