Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cytochrome b5 type B (CYB5B) Standard Curve.)
Human Cytochrome B5 Type B (CYB5B) ELISA Kit | CYB5B elisa kit
Human Cytochrome B5 Type B (CYB5B) ELISA Kit
Gene Names
CYB5B; OMB5; CYB5-M; CYPB5M
Reactivity
Human
Synonyms
Cytochrome B5 Type B (CYB5B); Human Cytochrome B5 Type B (CYB5B) ELISA Kit; CYB5B; CYB5-M; CYPB5M; DKFZp686M0619; OMB5; cytochrome b5 outer mitochondrial membrane; outer mitochondrial membrane cytochrome b5; type 2 cyt-b5; CYB5B elisa kit
Reactivity
Human
Specificity
Human Cytochrome b5 type B (CYB5B) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYB5B. No significant cross-reactivity or interference between Human CYB5B and analogues was observed.
Sequence Length
150
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cytochrome b5 type B (CYB5B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cytochrome b5 type B (CYB5B) Standard Curve.)
Related Product Information for CYB5B elisa kit
Description: This Human Cytochrome b5 type B (CYB5B) ELISA Kit employs a two-site sandwich ELISA to quantitate CYB5B in samples. An antibody specific for CYB5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYB5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: CYB5B (Cytochrome B5 Type B) is a Protein Coding gene. Among its related pathways are Metabolism and Cytochrome P450 - arranged by substrate type. GO annotations related to this gene include heme binding and enzyme activator activity. An important paralog of this gene is CYB5A. Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Background: CYB5B (Cytochrome B5 Type B) is a Protein Coding gene. Among its related pathways are Metabolism and Cytochrome P450 - arranged by substrate type. GO annotations related to this gene include heme binding and enzyme activator activity. An important paralog of this gene is CYB5A. Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cytochrome b5 type B
NCBI Official Synonym Full Names
cytochrome b5 type B
NCBI Official Symbol
CYB5B
NCBI Official Synonym Symbols
OMB5; CYB5-M; CYPB5M
NCBI Protein Information
cytochrome b5 type B
UniProt Protein Name
Cytochrome b5 type B
Protein Family
UniProt Gene Name
CYB5B
UniProt Synonym Gene Names
CYB5M; OMB5
UniProt Entry Name
CYB5B_HUMAN
Uniprot Description
CYB5B: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Belongs to the cytochrome b5 family.
Protein type: Membrane protein, integral; Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: mitochondrial outer membrane; membrane; integral to membrane
Molecular Function: metal ion binding; heme binding
Research Articles on CYB5B
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Product Notes
The Human CYB5B cyb5b (Catalog #AAA9715854) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715854 ELISA Kit recognizes Human CYB5B. It is sometimes possible for the material contained within the vial of "Cytochrome B5 Type B (CYB5B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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