Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Cytochrome b5 reductase 4 (CYB5R4) Standard Curve.)
Rat Cytochrome B5 Reductase 4 (CYB5R4) ELISA Kit | CYB5R4 elisa kit
Rat Cytochrome B5 Reductase 4 (CYB5R4) ELISA Kit
Gene Names
Cyb5r4; Ncb5or; b5&b5R; b5+b5R; cb5/cb5R
Reactivity
Rat
Synonyms
Cytochrome B5 Reductase 4 (CYB5R4); Rat Cytochrome B5 Reductase 4 (CYB5R4) ELISA Kit; CYB5R4; NCB5OR; RP4-676J13.1; dJ676J13.1; NADPH cytochrome B5 oxidoreductase; cytochrome b-type NAD(P)H oxidoreductase; flavohemoprotein b5+b5R; CYB5R4 elisa kit
Reactivity
Rat
Specificity
Rat Cytochrome b5 reductase 4 (CYB5R4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CYB5R4. No significant cross-reactivity or interference between Rat CYB5R4 and analogues was observed.
Sequence Length
520
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Cytochrome b5 reductase 4 (CYB5R4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Cytochrome b5 reductase 4 (CYB5R4) Standard Curve.)
Related Product Information for CYB5R4 elisa kit
Description: This Rat Cytochrome b5 reductase 4 (CYB5R4) ELISA Kit employs a two-site sandwich ELISA to quantitate CYB5R4 in samples. An antibody specific for CYB5R4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYB5R4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5R4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5R4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Cytochrome b5 reductase 4 is an enzyme that in humans is encoded by the CYB5R4 gene. NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5; MIM 250790) and CYB5 reductase.
Background: Cytochrome b5 reductase 4 is an enzyme that in humans is encoded by the CYB5R4 gene. NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5; MIM 250790) and CYB5 reductase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cytochrome b5 reductase 4
NCBI Official Synonym Full Names
cytochrome b5 reductase 4
NCBI Official Symbol
Cyb5r4
NCBI Official Synonym Symbols
Ncb5or; b5&b5R; b5+b5R; cb5/cb5R
NCBI Protein Information
cytochrome b5 reductase 4
UniProt Protein Name
Cytochrome b5 reductase 4
Protein Family
UniProt Gene Name
Cyb5r4
UniProt Synonym Gene Names
Ncb5or; b5+b5R
UniProt Entry Name
NB5R4_RAT
Research Articles on CYB5R4
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Product Notes
The Rat CYB5R4 cyb5r4 (Catalog #AAA9712489) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712489 ELISA Kit recognizes Rat CYB5R4. It is sometimes possible for the material contained within the vial of "Cytochrome B5 Reductase 4 (CYB5R4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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