Ki-67 Recombinant Protein | Ki67P recombinant protein

Recombinant Ki-67 Protein (Ki67P)

Cat. Num. AAA2029656

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Ki-67; Recombinant Ki-67 Protein (Ki67P); Ki67P recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 7.8 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MASSAHLVTI KRSGDDGAHF PLSLSSCLFG RSIECDIRIQ LPVVSKRHCK IEVKEQEAIL YNFSSTNPTQ VNGVTIDEPV RLRHGDIITI IDRSFRYEDG NHEDGSKPTE FPGKSLGKEP SRRASRDSFC ADPDGEGQDT KASKMTASRR SFVYAKGLSA DSPASDGSKN SVSQDSSGHV EQHTGRNIVE PTSGDLFKKSRSTGSSYREP KSSPTQSLSN SNEKESPFEK LYQSMKEELD VKSQKSC
• Sequence Length: 2938

• Applicable Applications for Ki67P recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 30.9kDa
• Accurate Molecular Mass (KD): 35kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Met1~Cys247
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 1177528
• UniProt Accession #: Q61769
• Molecular Weight: 324,429 Da
• NCBI Official Full Name: Ki-67
• UniProt Protein Name: Ki-67 protein
• UniProt Gene Name: Mki67
• UniProt Entry Name: Q61769_MOUSE

Uniprot Description

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Cell cycle regulation; Nucleolus

Cellular Component: chromosome, pericentric region; condensed chromosome; cytoplasm; membrane; nucleolus; nucleus

Molecular Function: protein C-terminus binding

Biological Process: cell proliferation; meiosis