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Mouse CD47 molecule ELISA Kit | CD47 elisa kit

Mouse Leukocyte surface antigen CD47, CD47 ELISA Kit

Gene Names
Cd47; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
Reactivity
Mouse
Synonyms
CD47 molecule; Mouse Leukocyte surface antigen CD47; CD47 ELISA Kit; Mouse Leukocyte surface antigen CD47 (CD47) ELISA kit; IAP; MER6; OA3; CD47 antigen; CD47 antigen (Rh-related antigen; integrin-associated signal transducer) ; CD47 glycoprotein; Rh-related antigen; antigen identified by monoclonal antibody 1D8; antigenic sur; CD47 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse CD47. No significant cross-reactivity or interference between mouse CD47 and analogues was observed.
Sequence Length
324
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
< 3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for CD47 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD47 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD47 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD47 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD47 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,098 Da
NCBI Official Full Name
leukocyte surface antigen CD47
NCBI Official Synonym Full Names
CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
NCBI Official Symbol
Cd47
NCBI Official Synonym Symbols
IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
NCBI Protein Information
leukocyte surface antigen CD47; Rh-related antigen; integrin-associated protein
UniProt Protein Name
Leukocyte surface antigen CD47
Protein Family
UniProt Gene Name
Cd47
UniProt Synonym Gene Names
IAP
UniProt Entry Name
CD47_MOUSE

Uniprot Description

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: cell migration; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; opsonization; positive regulation of T cell activation; signal transduction; cell adhesion; positive regulation of inflammatory response

Research Articles on CD47

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Product Notes

The Mouse CD47 cd47 (Catalog #AAA910495) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA910495 ELISA Kit recognizes Mouse CD47. It is sometimes possible for the material contained within the vial of "CD47 molecule, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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