Rabbit anti-Human ANAPC5 Polyclonal Antibody | anti-ANAPC5 antibody

ANAPC5, ID (ANAPC5, APC5, Anaphase-promoting complex subunit 5, Cyclosome subunit 5) (FITC)

Cat. Num. AAA6273436

• Gene Names: ANAPC5; APC5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ANAPC5; Polyclonal Antibody; ID (ANAPC5; APC5; Anaphase-promoting complex subunit 5; Cyclosome subunit 5) (FITC); Cyclosome subunit 5; anti-ANAPC5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ANAPC5 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ANAPC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the Central region of human ANAPC5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ANAPC5 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ANAPC5 antibody detected the ANAPC5 protein (arrow).)

Flow Cytometry (FC/FACS)

(ANAPC5 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ANAPC5 antibody

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Product Categories/Family for anti-ANAPC5 antibody

Antibodies; Cell Division Cycle (CDC)

References

Wasch, R., et al. Oncogene 29(1):1-10(2010). Jin, L., et al. Cell 133(4):653-665(2008). Liu, J., et al. Cancer Biol. Ther. 5(7):760-762(2006). Dube, P., et al. Mol. Cell 20(6):867-879(2005). Turnell, A.S., et al. Nature 438(7068):690-695(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 20127553
• NCBI GeneID: 51433
• NCBI Accession #: NP_057321.2
• NCBI GenBank Nucleotide #: NM_016237.4
• UniProt Accession #: Q9UJX4; Q8N4H7; Q9BQD4; E9PFB2
• Molecular Weight: 72,526 Da
• NCBI Official Full Name: anaphase-promoting complex subunit 5 isoform a
• NCBI Official Synonym Full Names: anaphase promoting complex subunit 5
• NCBI Official Symbol: ANAPC5
• NCBI Official Synonym Symbols: APC5
• NCBI Protein Information: anaphase-promoting complex subunit 5; cyclosome subunit 5
• UniProt Protein Name: Anaphase-promoting complex subunit 5
• UniProt Gene Name: ANAPC5
• UniProt Synonym Gene Names: APC5; APC5
• UniProt Entry Name: APC5_HUMAN

NCBI Description

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]

Uniprot Description

APC5: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol

Molecular Function: protein phosphatase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle