Chicken Macrophage inflammatory protein-1gamma ELISA Kit | MIP-1gamma elisa kit

Chicken Macrophage inflammatory protein-1gamma ELISA Kit

Cat. Num. AAA749693

• Gene Names: Ccl9; CCF18; MRP-2; Scya9; Scya10
• Reactivity: Chicken
• Synonyms: Macrophage inflammatory protein-1gamma; Chicken Macrophage inflammatory protein-1gamma ELISA Kit; Chicken Macrophage inflammatory protein-1g ELISA Kit; MIP-1gamma elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This MIP-1gamma ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken MIP-1gamma. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP-1gamma elisa kit

Principle of the Assay: MIP-1gamma ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MIP-1gamma antibody and an MIP-1gamma-HRP conjugate. The assay sample and buffer are incubated together with MIP-1gamma-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIP-1gamma concentration since MIP-1gamma from samples and MIP-1gamma-HRP conjugate compete for the anti-MIP-1gamma antibody binding site. Since the number of sites is limited, as more sites are occupied by MIP-1gamma from the sample, fewer sites are left to bind MIP-1gamma-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP-1gamma concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1234835
• NCBI GeneID: 20308
• Molecular Weight: 13,871 Da
• NCBI Official Full Name: macrophage inflammatory protein-1gamma
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 9
• NCBI Official Symbol: Ccl9
• NCBI Official Synonym Symbols: CCF18; MRP-2; Scya9; Scya10
• NCBI Protein Information: C-C motif chemokine 9; MIP-1 gamma; small inducible cytokine A9; small inducible cytokine A10; macrophage inflammatory protein 1-gamma; macrophage inflammatory protein-related protein 2
• UniProt Protein Name: C-C motif chemokine 9
• UniProt Gene Name: Ccl9
• UniProt Synonym Gene Names: Mrp2; Scya10; Scya9; MIP-1-gamma; MRP-2
• UniProt Entry Name: CCL9_MOUSE

Uniprot Description

CCL9: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors

Protein type: Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; cytokine activity

Biological Process: immune response; chemotaxis; negative regulation of myoblast differentiation