Human Lysophosphatidic acid receptor 1 ELISA Kit | LPAR1 elisa kit

Human Lysophosphatidic acid receptor 1 ELISA Kit

Cat. Num. AAA763386

• Gene Names: LPAR1; EDG2; LPA1; VZG1; GPR26; edg-2; vzg-1; Gpcr26; Mrec1.3; rec.1.3
• Reactivity: Human
• Synonyms: Lysophosphatidic acid receptor 1; Human Lysophosphatidic acid receptor 1 ELISA Kit; LPAR1/Lysophosphatidic acid receptor Edg-2/LPA receptor 1/LPA-1/EDG2/LPA1; LPAR1 elisa kit

• Reactivity: Human
• Sequence Length: 364

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LPAR1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human LPAR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LPAR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LPAR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LPAR1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16950638
• NCBI GeneID: 1902
• NCBI Accession #: NP_476500.1
• NCBI GenBank Nucleotide #: NM_057159.2
• Molecular Weight: 41,346 Da
• NCBI Official Full Name: lysophosphatidic acid receptor 1
• NCBI Official Synonym Full Names: lysophosphatidic acid receptor 1
• NCBI Official Symbol: LPAR1
• NCBI Official Synonym Symbols: EDG2; LPA1; VZG1; GPR26; edg-2; vzg-1; Gpcr26; Mrec1.3; rec.1.3
• NCBI Protein Information: lysophosphatidic acid receptor 1
• UniProt Protein Name: Lysophosphatidic acid receptor 1
• Protein Family: Lysophosphatidic acid receptor
• UniProt Gene Name: LPAR1
• UniProt Synonym Gene Names: EDG2; LPA1; LPA receptor 1; LPA-1
• UniProt Entry Name: LPAR1_HUMAN

NCBI Description

The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq, Jul 2008]

Uniprot Description

LPAR1: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 9q31.3

Cellular Component: cell soma; cell surface; cytosol; dendritic shaft; dendritic spine; endocytic vesicle; endosome; integral to plasma membrane; plasma membrane

Molecular Function: G-protein alpha-subunit binding; G-protein coupled receptor activity; PDZ domain binding; protein binding

Biological Process: activation of MAPK activity; bleb formation; cerebellum development; corpus callosum development; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; myelination; negative regulation of cAMP biosynthetic process; oligodendrocyte development; optic nerve development; phospholipase C activation; positive regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of MAPKKK cascade; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; regulation of cell shape