Typical Testing Data/Standard Curve (for reference only)
Mouse CCL9/Mip 1 Gamma ELISA Kit | CCL9 elisa kit
Mouse CCL9/Mip 1 Gamma PicoKine Fast ELISA Kit
Gene Names
Ccl9; CCF18; MRP-2; Scya9; Scya10
Reactivity
Mouse
Synonyms
CCL9/Mip 1 Gamma; Mouse CCL9/Mip 1 Gamma PicoKine Fast ELISA Kit; mouse CCL9; C-C motif chemokine 9; CCF18; Macrophage inflammatory protein 1-gamma; MIP-1-gamma; Macrophage inflammatory protein-related protein 2; MRP-2; Small-inducible cytokine A9; CCL9(29-101); CCL9(30-101); CCL9(31-101); Ccl9; Mrp2; Scya10; Scya9; CCL9 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Ccl9
Sequence Length
122
Samples
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
7.8pg/ml-500pg/ml
Sensitivity
<1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCL9 elisa kit
Principle of the Assay: The Picokineâ„¢ Mouse Ccl9 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Ccl9 with a 96-well strip plate that is pre-coated with antibody specific for Ccl9. The detection antibody is a biotinylated antibody specific for Ccl9. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Ccl9 with immunogen: Expression system for standard: E.coli; Immunogen sequence: Q22-Q122. The kit is analytically validated with ready to use reagents. To measure Mouse Ccl9, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Ccl9 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ccl9 in the sample.!!Background/Introduction: Chemokine(C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
macrophage inflammatory protein-1gamma
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 9
NCBI Official Symbol
Ccl9
NCBI Official Synonym Symbols
CCF18; MRP-2; Scya9; Scya10
NCBI Protein Information
C-C motif chemokine 9
UniProt Protein Name
C-C motif chemokine 9
Protein Family
UniProt Gene Name
Ccl9
UniProt Synonym Gene Names
Mrp2; Scya10; Scya9; MIP-1-gamma; MRP-2
UniProt Entry Name
CCL9_MOUSE
Uniprot Description
CCL9: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors
Protein type: Cytokine
Cellular Component: extracellular space; extracellular region
Molecular Function: chemokine activity; cytokine activity
Biological Process: immune response; chemotaxis; negative regulation of myoblast differentiation
Research Articles on CCL9
Similar Products
Product Notes
The Mouse CCL9 ccl9 (Catalog #AAA1752764) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752764 ELISA Kit recognizes Mouse CCL9. It is sometimes possible for the material contained within the vial of "CCL9/Mip 1 Gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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