Mouse CCL9/Mip 1 Gamma ELISA Kit | CCL9 elisa kit

Mouse CCL9/Mip 1 Gamma PicoKine Fast ELISA Kit

Cat. Num. AAA1752764

• Gene Names: Ccl9; CCF18; MRP-2; Scya9; Scya10
• Reactivity: Mouse
• Synonyms: CCL9/Mip 1 Gamma; Mouse CCL9/Mip 1 Gamma PicoKine Fast ELISA Kit; mouse CCL9; C-C motif chemokine 9; CCF18; Macrophage inflammatory protein 1-gamma; MIP-1-gamma; Macrophage inflammatory protein-related protein 2; MRP-2; Small-inducible cytokine A9; CCL9(29-101); CCL9(30-101); CCL9(31-101); Ccl9; Mrp2; Scya10; Scya9; CCL9 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Ccl9
• Sequence Length: 122

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 7.8pg/ml-500pg/ml
• Sensitivity: <1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL9 elisa kit

Principle of the Assay: The Picokine™ Mouse Ccl9 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Ccl9 with a 96-well strip plate that is pre-coated with antibody specific for Ccl9. The detection antibody is a biotinylated antibody specific for Ccl9. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Ccl9 with immunogen: Expression system for standard: E.coli; Immunogen sequence: Q22-Q122. The kit is analytically validated with ready to use reagents. To measure Mouse Ccl9, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Ccl9 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ccl9 in the sample.!!Background/Introduction: Chemokine(C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.

NCBI and Uniprot Product Information

• NCBI GI #: 1234835
• NCBI GeneID: 20308
• UniProt Accession #: P51670
• NCBI Official Full Name: macrophage inflammatory protein-1gamma
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 9
• NCBI Official Symbol: Ccl9
• NCBI Official Synonym Symbols: CCF18; MRP-2; Scya9; Scya10
• NCBI Protein Information: C-C motif chemokine 9
• UniProt Protein Name: C-C motif chemokine 9
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl9
• UniProt Synonym Gene Names: Mrp2; Scya10; Scya9; MIP-1-gamma; MRP-2
• UniProt Entry Name: CCL9_MOUSE

Uniprot Description

CCL9: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors

Protein type: Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; cytokine activity

Biological Process: immune response; chemotaxis; negative regulation of myoblast differentiation