Mouse CCL9 ELISA Kit | CCL9 elisa kit

Mouse CCL9 ELISA Kit

Cat. Num. AAA824740

• Gene Names: Ccl9; CCF18; MRP-2; Scya9; Scya10
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: CCL9; Mouse CCL9 ELISA Kit; Mrp2; Scya10; Scya9; C-C motif chemokine 9; CCF18; Macrophage inflammatory protein 1-gamma; MIP-1-gamma; Macrophage inflammatory protein-related protein 2; MRP-2; Small-inducible cytokine A9; CCL9 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse CCL21 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CCL21 proteins within the range of 31.2 pg/ml-2000 pg/ml.
• Sequence Length: 122

• Applicable Applications for CCL9 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL9 elisa kit

Principle of the Assay: The MyBioSource' Mouse CCL21 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CCL21 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CCL21 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CCL21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

NCBI and Uniprot Product Information

• NCBI GI #: 6755434
• NCBI GeneID: 20308
• NCBI Accession #: NP_035468.1
• NCBI GenBank Nucleotide #: NM_011338.2
• UniProt Accession #: P51670; Q5QNW2
• Molecular Weight: 13,871 Da
• NCBI Official Full Name: C-C motif chemokine 9
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 9
• NCBI Official Symbol: Ccl9
• NCBI Official Synonym Symbols: CCF18; MRP-2; Scya9; Scya10
• NCBI Protein Information: C-C motif chemokine 9; MIP-1 gamma; small inducible cytokine A9; small inducible cytokine A10; macrophage inflammatory protein 1-gamma; macrophage inflammatory protein-related protein 2
• UniProt Protein Name: C-C motif chemokine 9
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl9
• UniProt Synonym Gene Names: Mrp2; Scya10; Scya9; MIP-1-gamma; MRP-2
• UniProt Entry Name: CCL9_MOUSE

Uniprot Description

CCL9: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors

Protein type: Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; cytokine activity

Biological Process: immune response; chemotaxis; negative regulation of myoblast differentiation