Typical Testing Data/Standard Curve (for reference only)
Human thymus expressed chemokine, TECK ELISA Kit | TECK elisa kit
Human thymus expressed chemokine, TECK ELISA Kit
Gene Names
Ccl25; TECK; CKb15; Scya25; AI852536; A130072A22Rik
Reactivity
Human
Synonyms
thymus expressed chemokine; TECK; Human thymus expressed chemokine; TECK ELISA Kit; TECK elisa kit
Reactivity
Human
Sequence Length
144
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
30ng/ml-9000ng/ml
Sensitivity
15.79ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TECK elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Thymosin Beta 4 (also known as TMSB4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human TMSB4 antibody. TMSB4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TMSB4 Antibody is added and binds to TMSB4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TMSB4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TMSB4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human TMSB4 antibody. TMSB4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TMSB4 Antibody is added and binds to TMSB4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TMSB4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TMSB4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,733 Da
NCBI Official Full Name
thymus-expressed chemokine
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 25
NCBI Official Symbol
Ccl25
NCBI Official Synonym Symbols
TECK; CKb15; Scya25; AI852536; A130072A22Rik
NCBI Protein Information
C-C motif chemokine 25
UniProt Protein Name
C-C motif chemokine 25
UniProt Gene Name
Ccl25
UniProt Synonym Gene Names
Scya25; Teck
Uniprot Description
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Research Articles on TECK
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Product Notes
The Human TECK ccl25 (Catalog #AAA162437) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA162437 ELISA Kit recognizes Human TECK. It is sometimes possible for the material contained within the vial of "thymus expressed chemokine, TECK, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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