Typical Testing Data/Standard Curve (for reference only)
Rat TECK ELISA Kit | TECK elisa kit
Rat TECK (Thymus Expressed Chemokine) ELISA Kit
Gene Names
Ccl25; TECK; CKb15; Scya25; AI852536; A130072A22Rik
Reactivity
Rat
Synonyms
TECK; Rat TECK (Thymus Expressed Chemokine) ELISA Kit; TECK elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat TECK. No significant cross-reactivity or interference between Rat TECK and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat TECK and all the analogues, therefore, cross reaction may still exist.
Sequence Length
144
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TECK elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TECK in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TECK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TECK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TECK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat TECK. You can calculate the concentration of Rat TECK in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TECK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TECK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TECK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat TECK. You can calculate the concentration of Rat TECK in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,733 Da
NCBI Official Full Name
thymus-expressed chemokine
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 25
NCBI Official Symbol
Ccl25
NCBI Official Synonym Symbols
TECK; CKb15; Scya25; AI852536; A130072A22Rik
NCBI Protein Information
C-C motif chemokine 25; small inducible chemokine 25; small inducible cytokine A25; thymus-expressed chemokine
UniProt Protein Name
C-C motif chemokine 25
UniProt Gene Name
Ccl25
UniProt Synonym Gene Names
Scya25; Teck
UniProt Entry Name
CCL25_MOUSE
Uniprot Description
CCL25: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Belongs to the intercrine beta (chemokine CC) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; cytokine activity
Biological Process: G-protein coupled receptor protein signaling pathway; immune response; chemotaxis; positive regulation of cell-matrix adhesion; inflammatory response; leukocyte migration
Research Articles on TECK
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Product Notes
The Rat TECK ccl25 (Catalog #AAA2509259) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509259 ELISA Kit recognizes Rat TECK. It is sometimes possible for the material contained within the vial of "TECK, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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