Mouse Cathepsin G (CTSG) ELISA Kit | CTSG elisa kit

Mouse Cathepsin G (CTSG) ELISA Kit

Cat. Num. AAA2019170

• Gene Names: CTSG; CG; CATG
• Reactivity: Mouse
• Synonyms: Cathepsin G (CTSG); Mouse Cathepsin G (CTSG) ELISA Kit; CG; CTS-G; CTSG elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin G (CTSG).; No significant cross-reactivity or interference between Cathepsin G (CTSG) and analogues was observed.
• Sequence Length: 255

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin G (CTSG) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin G (CTSG) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSG elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin G (CTSG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin G (CTSG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin G (CTSG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin G (CTSG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CTSG elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4503149
• NCBI GeneID: 1511
• NCBI Accession #: NP_001902.1
• NCBI GenBank Nucleotide #: NM_001911.2
• UniProt Accession #: P08311; Q6IBJ6; Q9UCA5; Q9UCU6
• Molecular Weight: 28,837 Da
• NCBI Official Full Name: cathepsin G preproprotein
• NCBI Official Synonym Full Names: cathepsin G
• NCBI Official Symbol: CTSG
• NCBI Official Synonym Symbols: CG; CATG
• NCBI Protein Information: cathepsin G
• UniProt Protein Name: Cathepsin G
• Protein Family: Cathepsin
• UniProt Gene Name: CTSG
• UniProt Synonym Gene Names: CG
• UniProt Entry Name: CATG_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family.

Protein type: Motility/polarity/chemotaxis; EC 3.4.21.20; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular matrix; extracellular space; cell surface; extracellular region; plasma membrane; nucleus; secretory granule

Molecular Function: peptidase activity; heparin binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; positive regulation of immune response; cellular protein metabolic process; protein processing; response to lipopolysaccharide; immune response; proteolysis; angiotensin maturation; defense response to fungus