Human Cathepsin G ELISA Kit | CTSG elisa kit

Human Cathepsin G ELISA Kit

Cat. Num. AAA2881992

• Gene Names: CTSG; CG; CATG
• Reactivity: Human
• Synonyms: Cathepsin G; Human Cathepsin G ELISA Kit; CG; CTSG; 3.4.21.20; CTSG elisa kit

• Reactivity: Human
• Sequence Length: 255

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSG elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503149
• NCBI GeneID: 1511
• NCBI Accession #: NP_001902.1
• NCBI GenBank Nucleotide #: NM_001911.2
• UniProt Accession #: P08311; Q6IBJ6; Q9UCA5; Q9UCU6
• Molecular Weight: 28,837 Da
• NCBI Official Full Name: cathepsin G preproprotein
• NCBI Official Synonym Full Names: cathepsin G
• NCBI Official Symbol: CTSG
• NCBI Official Synonym Symbols: CG; CATG
• NCBI Protein Information: cathepsin G
• UniProt Protein Name: Cathepsin G
• Protein Family: Cathepsin
• UniProt Gene Name: CTSG
• UniProt Synonym Gene Names: CG
• UniProt Entry Name: CATG_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family.

Protein type: Motility/polarity/chemotaxis; EC 3.4.21.20; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: cell surface; extracellular matrix; extracellular region; extracellular space; nucleus; plasma membrane; secretory granule

Molecular Function: heparin binding; peptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: angiotensin maturation; cellular protein metabolic process; defense response to fungus; extracellular matrix disassembly; extracellular matrix organization and biogenesis; immune response; positive regulation of immune response; protein amino acid phosphorylation; protein processing; proteolysis; response to lipopolysaccharide