Typical Testing Data/Standard Curve (for reference only)
Porcine Cathepsin G (cath-G) ELISA Kit | CTSG elisa kit
Porcine Cathepsin G (cath-G) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CTSE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CTSE antibody and an CTSE-HRP conjugate. The assay sample and buffer are incubated together with CTSE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTSE concentration since CTSE from samples and CTSE-HRP conjugate compete for the anti-CTSE antibody binding site. Since the number of sites is limited, as more sites are occupied by CTSE from the sample, fewer sites are left to bind CTSE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTSE concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family.
Protein type: Motility/polarity/chemotaxis; EC 3.4.21.20; Protease
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: extracellular matrix; extracellular space; cell surface; extracellular region; plasma membrane; nucleus; secretory granule
Molecular Function: heparin binding; peptidase activity; serine-type endopeptidase activity
Biological Process: extracellular matrix disassembly; positive regulation of immune response; extracellular matrix organization and biogenesis; cellular protein metabolic process; protein processing; response to lipopolysaccharide; immune response; proteolysis; angiotensin maturation; defense response to fungus
Research Articles on CTSG
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Product Notes
The Porcine CTSG ctsg (Catalog #AAA7236421) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7236421 ELISA Kit recognizes Porcine CTSG. It is sometimes possible for the material contained within the vial of "Cathepsin G (cath-G), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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