Human cathepsin G ELISA Kit | CTSG elisa kit

Human Cathepsin G, Cath-G ELISA Kit

Cat. Num. AAA705796

• Gene Names: CTSG; CG; CATG
• Reactivity: Human
• Synonyms: cathepsin G; Human Cathepsin G; Cath-G ELISA Kit; Human Cathepsin G (cath-G) ELISA Kit; CG; MGC23078; ; CTSG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human cath-G. No significant cross-reactivity or interference between human cath-G and analogues was observed.
• Sequence Length: 255

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml
• Sensitivity: Typically less than 7.8 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSG elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cath-G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cath-G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cath-G is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cath-G bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503149
• NCBI GeneID: 1511
• NCBI Accession #: NP_001902.1
• NCBI GenBank Nucleotide #: NM_001911.2
• UniProt Accession #: P08311; Q6IBJ6; Q9UCA5; Q9UCU6
• Molecular Weight: 28,837 Da
• NCBI Official Full Name: cathepsin G preproprotein
• NCBI Official Synonym Full Names: cathepsin G
• NCBI Official Symbol: CTSG
• NCBI Official Synonym Symbols: CG; CATG
• NCBI Protein Information: cathepsin G
• UniProt Protein Name: Cathepsin G
• Protein Family: Cathepsin
• UniProt Gene Name: CTSG
• UniProt Synonym Gene Names: CG
• UniProt Entry Name: CATG_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride.3 PublicationsManual assertion based on experiment in:Ref.6

Catalytic activity: Specificity similar to chymotrypsin C.

Enzyme regulation: Inhibited by soybean trypsin inhibitor, benzamidine, the synthetic peptide R13K, Z-Gly-Leu-Phe-CH2Cl, phenylmethylsulfonyl fluoride, 3,4-dichloroisocoumarin, DFP, SBTI and alpha-1-antitrypsin. Inhibited by LPS from P.aeruginosa but not by LPS from S.minnesota. Not inhibited by elastinal, CMK, TLCK and ETDA.3 PublicationsManual assertion based on experiment in:Ref.6