Mouse Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit | ARNT elisa kit

Mouse Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit

Cat. Num. AAA452782

• Gene Names: Arnt; Drnt; Hif1b; ESTM42; W08714; bHLHe2; mKIAA4051; D3Ertd557e
• Reactivity: Mouse
• Synonyms: Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT); Mouse Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit; HIF-1beta; bHLHe2; Hypoxia Inducible Factor 1 Beta; Class E basic helix-loop-helix protein 2; Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta; ARNT elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ARNT. No significant cross-reactivity or interference between ARNT and analogues was observed.
• Sequence Length: 791

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ARNT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ARNT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARNT elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ARNT in mouse tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ARNT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ARNT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ARNT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ARNT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 83627709
• NCBI GeneID: 11863
• NCBI Accession #: NP_001032826.1
• NCBI GenBank Nucleotide #: NM_001037737.2
• UniProt Accession #: P53762; Q60661; Q921F3
• Molecular Weight: 84,613 Da
• NCBI Official Full Name: aryl hydrocarbon receptor nuclear translocator isoform a
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor nuclear translocator
• NCBI Official Symbol: Arnt
• NCBI Official Synonym Symbols: Drnt; Hif1b; ESTM42; W08714; bHLHe2; mKIAA4051; D3Ertd557e
• NCBI Protein Information: aryl hydrocarbon receptor nuclear translocator
• UniProt Protein Name: Aryl hydrocarbon receptor nuclear translocator
• Protein Family: Aryl hydrocarbon receptor nuclear translocator
• UniProt Gene Name: Arnt
• UniProt Synonym Gene Names: ARNT protein; HIF-1-beta; HIF1-beta

Uniprot Description

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia ().