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Typical Testing Data/Standard Curve (for reference only)

Human Beta-1,4-galactosyltransferase 5 ELISA Kit | B4GALT5 elisa kit

Human Beta-1,4-galactosyltransferase 5 ELISA Kit

Gene Names
B4GALT5; gt-V; B4Gal-T5; beta4Gal-T5; beta4GalT-V; BETA4-GALT-IV
Reactivity
Human
Synonyms
Beta-1; 4-galactosyltransferase 5; Human Beta-1; 4-galactosyltransferase 5 ELISA Kit; B4GALT5/Beta-1; 4-GalTase 5/Beta4Gal-T5/b4Gal-T5/UDP-galactose:beta-N-acetylglucosamine beta-1; 4-galactosyltransferase 5/Beta-1; 4-GalT II/UDP-Gal:beta-GlcNAc beta-1; B4GALT5 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of B4GALT5. No significant cross-reactivity or interference between B4GALT5 and analogues was observed.
Sequence Length
388
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for B4GALT5 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,119 Da
NCBI Official Full Name
beta-1,4-galactosyltransferase 5
NCBI Official Synonym Full Names
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5
NCBI Official Symbol
B4GALT5
NCBI Official Synonym Symbols
gt-V; B4Gal-T5; beta4Gal-T5; beta4GalT-V; BETA4-GALT-IV
NCBI Protein Information
beta-1,4-galactosyltransferase 5
UniProt Protein Name
Beta-1,4-galactosyltransferase 5
UniProt Gene Name
B4GALT5
UniProt Synonym Gene Names
Beta-1,4-GalTase 5; Beta4Gal-T5; b4Gal-T5
UniProt Entry Name
B4GT5_HUMAN

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as beta4GalT2. [provided by RefSeq, Jul 2008]

Uniprot Description

B4GALT5: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Belongs to the glycosyltransferase 7 family.

Protein type: Membrane protein, integral; Transferase; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 20q13.1-q13.2

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: galactosyltransferase activity; metal ion binding

Biological Process: carbohydrate metabolic process; cellular protein metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; keratan sulfate metabolic process; O-glycan processing; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; protein amino acid O-linked glycosylation

Research Articles on B4GALT5

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Product Notes

The Human B4GALT5 b4galt5 (Catalog #AAA765497) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA765497 ELISA Kit recognizes Human B4GALT5. It is sometimes possible for the material contained within the vial of "Beta-1,4-galactosyltransferase 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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