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Typical Testing Data/Standard Curve (for reference only)

Human Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) RTU ELISA Kit | ARNT rtu elisa kit

Human Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit

Gene Names
ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
Reactivity
Human
Synonyms
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT); Human Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit; HIF-1beta; bHLHe2; Hypoxia Inducible Factor 1 Beta; Class E basic helix-loop-helix protein 2; Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta; ARNT rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
773
Assay Type
Sandwich
Samples
Tissue Homogenates, Cell Lysates or Other Biological Fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.067ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ARNT rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ARNT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ARNT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ARNT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ARNT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
405
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86,366 Da
NCBI Official Full Name
aryl hydrocarbon receptor nuclear translocator isoform 4
NCBI Official Synonym Full Names
aryl hydrocarbon receptor nuclear translocator
NCBI Official Symbol
ARNT
NCBI Official Synonym Symbols
HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
NCBI Protein Information
aryl hydrocarbon receptor nuclear translocator
UniProt Protein Name
Aryl hydrocarbon receptor nuclear translocator
UniProt Gene Name
ARNT
UniProt Synonym Gene Names
BHLHE2; ARNT protein; bHLHe2; HIF-1-beta; HIF1-beta

NCBI Description

This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]

Uniprot Description

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.

Research Articles on ARNT

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Product Notes

The Human ARNT arnt (Catalog #AAA457764) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA457764 RTU ELISA Kit recognizes Human ARNT. It is sometimes possible for the material contained within the vial of "Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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