Human Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit | ARNT elisa kit

Human Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit

Cat. Num. AAA2705125

• Gene Names: ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
• Reactivity: Human
• Synonyms: Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT); Human Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit; HIF-1beta; bHLHe2; Hypoxia Inducible Factor 1 Beta; Class E basic helix-loop-helix protein 2; Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta; ARNT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT).; No significant cross-reactivity or interference between Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) and analogues was observed.
• Sequence Length: 50

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARNT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 338522013
• NCBI GeneID: 405
• Molecular Weight: 6,006 Da
• NCBI Official Full Name: aryl hydrocarbon receptor nuclear translocator, partial
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor nuclear translocator
• NCBI Official Symbol: ARNT
• NCBI Official Synonym Symbols: HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
• NCBI Protein Information: aryl hydrocarbon receptor nuclear translocator
• UniProt Protein Name: Aryl hydrocarbon receptor nuclear translocator
• Protein Family: Aryl hydrocarbon receptor nuclear translocator
• UniProt Gene Name: ARNT

NCBI Description

This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]