Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Adenosine receptor A2a (ADORA2A) Standard Curve.)
Mouse Adenosine Receptor A2a (ADORA2A) ELISA Kit | ADORA2A elisa kit
Mouse Adenosine Receptor A2a (ADORA2A) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Adenosine receptor A2a (ADORA2A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Adenosine receptor A2a (ADORA2A) Standard Curve.)
Background: ADORA2a is a member of the G protein-coupled receptor (GPCR) family which possess seven transmembrane alpha helices. The crystallographic structure of the adenosine A2A receptor PDB 3EML (see figure to the right) reveals a ligand binding pocket distinct from that of other structurally determined GPCRs. Adenosine A2a Receptoris one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein-coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.As with the A1, the A2A receptors are believed to play a role in regulating myocardial oxygen consumption and coronary blood flow.
NCBI and Uniprot Product Information
Uniprot Description
ADORA2A: one of several receptor subtypes for adenosine. A G-protein coupled receptor. Activation is mediated by G proteins which activate adenylyl cyclase. Abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1
Cellular Component: integral to plasma membrane; postsynaptic density; dendrite; integral to membrane; intermediate filament; endomembrane system; axolemma; presynaptic membrane; asymmetric synapse; postsynaptic membrane; membrane; cell soma; axon; presynaptic active zone; plasma membrane
Molecular Function: G-protein coupled receptor activity; identical protein binding; signal transducer activity; enzyme binding; protein heterodimerization activity; type 5 metabotropic glutamate receptor binding; alpha-actinin binding; adenosine receptor activity, G-protein coupled
Biological Process: response to alkaloid; positive regulation of circadian sleep/wake cycle, sleep; synaptic transmission, dopaminergic; prepulse inhibition; positive regulation of glutamate secretion; regulation of synaptic plasticity; locomotory behavior; negative regulation of caspase activity; signal transduction; vasodilation; G-protein signaling, adenylate cyclase activating pathway; positive regulation of synaptic transmission, glutamatergic; synaptic transmission, cholinergic; response to caffeine; regulation of inhibitory postsynaptic membrane potential; negative regulation of cell proliferation; negative regulation of alpha-beta T cell activation; negative regulation of locomotion; regulation of transcription, DNA-dependent; regulation of mitochondrial membrane potential; positive regulation of cAMP biosynthetic process; protein kinase C activation; negative regulation of neuron apoptosis; adenosine receptor signaling pathway; positive regulation of acetylcholine secretion; synaptic transmission, glutamatergic; eating behavior; response to amphetamine; positive regulation of synaptic transmission, GABAergic; regulation of calcium ion transport; regulation of norepinephrine secretion; membrane depolarization; G-protein coupled receptor protein signaling pathway; positive regulation of protein secretion; negative regulation of inflammatory response; negative regulation of vascular permeability; negative regulation of protein kinase activity; regulation of excitatory postsynaptic membrane potential; astrocyte activation; neurite morphogenesis
Research Articles on ADORA2A
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Product Notes
The Mouse ADORA2A adora2a (Catalog #AAA9717170) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717170 ELISA Kit recognizes Mouse ADORA2A. It is sometimes possible for the material contained within the vial of "Adenosine Receptor A2a (ADORA2A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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