Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Adenosine receptor A3 (ADORA3) Standard Curve.)
Rat Adenosine Receptor A3 (ADORA3) ELISA Kit | ADORA3 elisa kit
Rat Adenosine Receptor A3 (ADORA3) ELISA Kit
Reactivity
Rat
Synonyms
Adenosine Receptor A3 (ADORA3); Rat Adenosine Receptor A3 (ADORA3) ELISA Kit; ADORA3; RP11-552M11.7; A3AR; AD026; bA552M11.5; ADORA3 elisa kit
Reactivity
Rat
Specificity
Rat Adenosine receptor A3 (ADORA3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ADORA3. No significant cross-reactivity or interference between Rat ADORA3 and analogues was observed.
Sequence Length
319
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Adenosine receptor A3 (ADORA3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Adenosine receptor A3 (ADORA3) Standard Curve.)
Related Product Information for ADORA3 elisa kit
Description: This Rat Adenosine receptor A3 (ADORA3) ELISA Kit employs a two-site sandwich ELISA to quantitate ADORA3 in samples. An antibody specific for ADORA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADORA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The receptor is an adenosine receptor, but also denotes the human gene encoding it.Adenosine A3 receptors are G protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. It mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death.An adenosine A3 receptor agonist (CF-101) is in clinical trials for the treatment of rheumatoid arthritis. In a mouse model of infarction the A3 selective agonist CP-532,903 protected against myocardial ischemia and reperfusion injury.
Background: The receptor is an adenosine receptor, but also denotes the human gene encoding it.Adenosine A3 receptors are G protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. It mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death.An adenosine A3 receptor agonist (CF-101) is in clinical trials for the treatment of rheumatoid arthritis. In a mouse model of infarction the A3 selective agonist CP-532,903 protected against myocardial ischemia and reperfusion injury.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Adenosine receptor A3
NCBI Official Symbol
ADORA3
NCBI Protein Information
adenosine receptor A3
UniProt Protein Name
Adenosine receptor A3
Protein Family
UniProt Gene Name
ADORA3
Uniprot Description
Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.
Research Articles on ADORA3
Similar Products
Product Notes
The Rat ADORA3 adora3 (Catalog #AAA9711971) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711971 ELISA Kit recognizes Rat ADORA3. It is sometimes possible for the material contained within the vial of "Adenosine Receptor A3 (ADORA3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
