Human Adenosine A2a Receptor (ADORA2a) RTU ELISA Kit | ADORA2a rtu elisa kit

Human Adenosine A2a Receptor (ADORA2a) ELISA Kit

Cat. Num. AAA457425

• Gene Names: ADORA2A; A2aR; RDC8; ADORA2
• Reactivity: Human
• Synonyms: Adenosine A2a Receptor (ADORA2a); Human Adenosine A2a Receptor (ADORA2a) ELISA Kit; ADORA2; RDC8; hA2AR; ADORA2a rtu elisa kit

• Reactivity: Human
• Sequence Length: 412

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.060ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADORA2a rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADORA2a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADORA2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADORA2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADORA2a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5921992
• NCBI GeneID: 135
• NCBI Accession #: NP_000666.2
• NCBI GenBank Nucleotide #: NM_000675.5
• UniProt Accession #: P29274; B2R7E0
• Molecular Weight: 44,707 Da
• NCBI Official Full Name: adenosine receptor A2a
• NCBI Official Synonym Full Names: adenosine A2a receptor
• NCBI Official Symbol: ADORA2A
• NCBI Official Synonym Symbols: A2aR; RDC8; ADORA2
• NCBI Protein Information: adenosine receptor A2a
• UniProt Protein Name: Adenosine receptor A2a
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA2A
• UniProt Synonym Gene Names: ADORA2

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

Uniprot Description

Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.