CD3/CD19 Dual Color Reagent | CD3 dual color reagent

MOUSE ANTI HUMAN CD3:FITC/CD19:RPE

Cat. Num. AAA238059

• Gene Names: CD3G; T3G; IMD17; CD3-GAMMA
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD3/CD19; MOUSE ANTI HUMAN CD3:FITC/CD19:RPE; CD3 dual color reagent

• Host: Mouse
• Isotype: FITC reagent: IgG1 (MOUSE) ; RPE reagent: IgG1 (MOUSE)
• Clone Number: UCHT1/LT19
• Specificity: UCHT1 recognises the human CD3 antigen, a complex of 5 polypeptide chains with molecular weights of 16-28kD, expressed by mature T cells.LT19 recognises the human CD19 antigen, a pan B cell marker. ; ; This item may be used to simultaneously enumerate CD3 and CD19 positive cells.
• Form/Format: FITC/RPE; Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised
• Sequence Length: 182

• Applicable Applications for CD3 dual color reagent: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood ; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 0.5 ml distilled water; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(MBS238059 staining of human lymphocytes)

Related Product Information for CD3 dual color reagent

UCHT1 recognises the human CD3 antigen, a complex of 5 polypeptide chains with molecular weights of 16-28kD, expressed by mature T cells. LT19 recognises the human CD19 antigen, a pan B cell marker. MBS238059 may be used to simultaneously enumerate CD3 and CD19 positive cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4557429
• NCBI GeneID: 917
• NCBI Accession #: NP_000064.1
• NCBI GenBank Nucleotide #: NM_000073.2
• Molecular Weight: 20,469 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 gamma chain
• NCBI Official Synonym Full Names: CD3g molecule, gamma (CD3-TCR complex)
• NCBI Official Symbol: CD3G
• NCBI Official Synonym Symbols: T3G; IMD17; CD3-GAMMA
• NCBI Protein Information: T-cell surface glycoprotein CD3 gamma chain; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 gamma chain
• UniProt Gene Name: CD3G
• UniProt Synonym Gene Names: T3G
• UniProt Entry Name: CD3G_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3G: The CD3 complex mediates signal transduction.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; alpha-beta T cell receptor complex

Molecular Function: transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; T cell receptor binding

Biological Process: protein transport; regulation of immune response; cell surface receptor linked signal transduction; T cell activation; T cell costimulation; establishment and/or maintenance of cell polarity; innate immune response; protein complex assembly; T cell receptor signaling pathway

Disease: Immunodeficiency 17