Mouse Tissue Factor (TF) CLIA Kit | TF clia kit

Mouse Tissue Factor (TF) CLIA Kit

Cat. Num. AAA2708775

• Gene Names: F3; TF; Cf3; Cf-3; CD142; AA409063
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Tissue Factor (TF); Mouse Tissue Factor (TF) CLIA Kit; Tissue Factor; CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III; TF clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tissue Factor (TF).; No significant cross-reactivity or interference between Tissue Factor (TF) and analogues was observed.
• Sequence Length: 294

• Applicable Applications for TF clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 2.74-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.95pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor (TF) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor (TF) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Tissue Factor (TF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tissue Factor (TF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tissue Factor (TF) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 170172540
• NCBI GeneID: 14066
• NCBI Accession #: NP_034301.3
• NCBI GenBank Nucleotide #: NP_034301.3
• UniProt Accession #: P20352
• NCBI Official Full Name: tissue factor
• NCBI Official Synonym Full Names: coagulation factor III
• NCBI Official Symbol: F3
• NCBI Official Synonym Symbols: TF; Cf3; Cf-3; CD142; AA409063
• NCBI Protein Information: tissue factor
• UniProt Protein Name: Tissue factor
• Protein Family: Tissue factor
• UniProt Gene Name: F3
• UniProt Synonym Gene Names: Cf-3; Cf3; TF

NCBI Description

This gene encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. The encoded protein binds to coagulation factor VIIa and the ensuing complex catalyzes the proteolytic activation of coagulation factors IX and X. Mice lacking encoded protein die in utero resulting from massive hemorrhaging in both extraembryonic and embryonic vessels. A severe deficiency of the encoded protein in mice results in impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis. [provided by RefSeq, Aug 2015]

Uniprot Description

F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3 G1|3 52.94 cM

Cellular Component: cell surface; cytoplasm; extracellular matrix; extracellular space; integral to membrane; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; phospholipid binding; protease binding; serine-type endopeptidase activity

Biological Process: activation of plasma proteins during acute inflammatory response; blood coagulation; caspase activation; cytokine and chemokine mediated signaling pathway; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell migration; protein processing