Rabbit MAP3K2 Polyclonal Antibody | anti-MAP3K2 antibody

MAP3K2 Polyclonal Antibody

Cat. Num. AAA3006352

• Gene Names: MAP3K2; MEKK2; MEKK2B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAP3K2; Polyclonal Antibody; MAP3K2 Polyclonal Antibody; Mitogen-activated protein kinase kinase kinase 2; EC=2.7.11.25; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK2; MAPKKK2; anti-MAP3K2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAP3K2 polyclonal antibody detects endogenous levels of MAP3K2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 619

• Applicable Applications for anti-MAP3K2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MAP3K2
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MAP3K2 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-MAP3K2 antibody

Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 97536681
• NCBI GeneID: 10746
• UniProt Accession #: Q9Y2U5
• Molecular Weight: ~ 75kDa
• NCBI Official Full Name: Mitogen-activated protein kinase kinase kinase 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 2
• NCBI Official Symbol: MAP3K2
• NCBI Official Synonym Symbols: MEKK2; MEKK2B
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 2
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 2
• UniProt Gene Name: MAP3K2
• UniProt Synonym Gene Names: MAPKKK2; MEKK2; MEK kinase 2; MEKK 2
• UniProt Entry Name: M3K2_HUMAN

NCBI Description

The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008]

Uniprot Description

MEKK2: a protein kinase of the STE11 family. Phosphorylates and activates MEK5. Involved in growth factor stimulated cell proliferation and differentiation.

Protein type: Kinase, protein; Protein kinase, STE; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Chromosomal Location of Human Ortholog: 2q14.3

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: activation of MAPKK activity; activation of MAPK activity; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; activation of JNK activity