Mouse IL-34 (Interleukin 34) CLIA Kit | IL-34 clia kit

Mouse IL-34 (Interleukin 34) CLIA Kit

Cat. Num. AAA2530922

• Gene Names: IL34; IL-34; C16orf77
• Reactivity: Mouse
• Synonyms: IL-34 (Interleukin 34); Mouse IL-34 (Interleukin 34) CLIA Kit; IL34; IL-34 clia kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant IL-34. No significant cross-reactivity or interference between IL-34 and analogues was observed.
• Sequence Length: 242

• Assay Type: Sandwich
• Detection Range: 12.5-800pg/mL
• Sensitivity: Min: 7.5pg/mL; Max: 800pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-34 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of IL-34 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to IL-34. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-34 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-34, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of IL-34. You can calculate the concentration of IL-34 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 289666752
• NCBI GeneID: 146433
• NCBI Accession #: NP_001166243.1
• NCBI GenBank Nucleotide #: NM_001172772.1
• Molecular Weight: 27,354 Da
• NCBI Official Full Name: interleukin-34 isoform 1
• NCBI Official Synonym Full Names: interleukin 34
• NCBI Official Symbol: IL34
• NCBI Official Synonym Symbols: IL-34; C16orf77
• NCBI Protein Information: interleukin-34
• UniProt Protein Name: Interleukin-34
• UniProt Gene Name: IL34
• UniProt Synonym Gene Names: C16orf77; IL-34
• UniProt Entry Name: IL34_HUMAN

NCBI Description

Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]

Uniprot Description

IL34: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Belongs to the IL-34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; positive regulation of cell proliferation; innate immune response; positive regulation of protein amino acid phosphorylation; inflammatory response; positive regulation of macrophage differentiation