anti-Human, Mouse POFUT2 Antibody | anti-POFUT2 antibody

POFUT2 Antibody

Cat. Num. AAA8509869

• Gene Names: POFUT2; FUT13; C21orf80
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: POFUT2; POFUT2 Antibody; GDP-fucose protein O-fucosyltransferase 2; EC 2.4.1.221; Peptide-O-fucosyltransferase 2; O-FucT-2; anti-POFUT2 antibody

• Reactivity: Human, Mouse
• Specificity: POFUT2 antibody detects endogenous levels of total POFUT2 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 424

• Applicable Applications for anti-POFUT2 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human POFUT2.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemistry analysis of paraffin-embedded human placenta tissue using POFUT2 antibody.)

References

Menzel O., Genomics 84:320-330(2004). Martinez-Duncker I., Glycobiology 13:1C-5C(2003). Nagase T., DNA Res. 6:63-70(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 13273311
• NCBI GeneID: 23275
• NCBI Accession #: NP_056042.1
• NCBI GenBank Nucleotide #: NM_015227.5
• UniProt Accession #: Q9Y2G5; Q6PJV1; Q7Z4N0; Q8WWU6; Q9BQS4; Q9BQS5; Q9UFY3
• Molecular Weight: 44,083 Da
• NCBI Official Full Name: GDP-fucose protein O-fucosyltransferase 2 isoform A
• NCBI Official Synonym Full Names: protein O-fucosyltransferase 2
• NCBI Official Symbol: POFUT2
• NCBI Official Synonym Symbols: FUT13; C21orf80
• NCBI Protein Information: GDP-fucose protein O-fucosyltransferase 2
• UniProt Protein Name: GDP-fucose protein O-fucosyltransferase 2
• UniProt Gene Name: POFUT2
• UniProt Synonym Gene Names: C21orf80; FUT13; KIAA0958; O-FucT-2

NCBI Description

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008]

Uniprot Description

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X2-G of thrombospondin type 1 repeats where C1 and C2 are the first and second cysteines, respectively. O-fucosylates members of several protein families including the ADAMTS family, the thrombosporin (TSP) and spondin families. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (). Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13.