Goat Prostaglandin E Metabolite ELISA Kit | PGEM elisa kit

Goat Prostaglandin E Metabolite (PGEM) ELISA Kit

Cat. Num. AAA2605321

• Gene Names: PTGER2; EP2
• Reactivity: Goat
• Synonyms: Prostaglandin E Metabolite; Goat Prostaglandin E Metabolite (PGEM) ELISA Kit; PGEM elisa kit

• Reactivity: Goat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 358

• Samples: Goat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGEM elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Goat PGEM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 31881630
• NCBI GeneID: 5732
• NCBI Accession #: NP_000947.2
• NCBI GenBank Nucleotide #: NM_000956.3
• Molecular Weight: 39,761 Da
• NCBI Official Full Name: prostaglandin E2 receptor EP2 subtype
• NCBI Official Synonym Full Names: prostaglandin E receptor 2
• NCBI Official Symbol: PTGER2
• NCBI Official Synonym Symbols: EP2
• NCBI Protein Information: prostaglandin E2 receptor EP2 subtype
• UniProt Protein Name: Prostaglandin E2 receptor EP2 subtype
• UniProt Gene Name: PTGER2
• UniProt Synonym Gene Names: PGE receptor EP2 subtype; PGE2 receptor EP2 subtype
• UniProt Entry Name: PE2R2_HUMAN

NCBI Description

This gene encodes a receptor for prostaglandin E2, a metabolite of arachidonic acid which has different biologic activities in a wide range of tissues. Mutations in this gene are associated with aspirin-induced susceptibility to asthma. [provided by RefSeq, Oct 2009]

Uniprot Description

PTGER2: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 14q22

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: prostaglandin E receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; regulation of cell proliferation; response to lipopolysaccharide; response to progesterone stimulus

Disease: Asthma, Nasal Polyps, And Aspirin Intolerance