Rat IL-34 ELISA Kit | IL-34 elisa kit

Rat IL-34 (Interleukin 34) ELISA Kit

Cat. Num. AAA2515815

• Gene Names: IL34; IL-34; C16orf77
• Reactivity: Rat
• Synonyms: IL-34; Rat IL-34 (Interleukin 34) ELISA Kit; IL-34 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat IL-34. No significant cross-reactivity or interference between Rat IL-34 and analogues was observed.
• Sequence Length: 242

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IL-34 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-34 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-34. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-34 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-34, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-34. You can calculate the concentration of IL-34 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 289666752
• NCBI GeneID: 146433
• NCBI Accession #: NP_001166243.1
• NCBI GenBank Nucleotide #: NM_001172772.1
• Molecular Weight: 27,354 Da
• NCBI Official Full Name: interleukin-34 isoform 1
• NCBI Official Synonym Full Names: interleukin 34
• NCBI Official Symbol: IL34
• NCBI Official Synonym Symbols: IL-34; C16orf77
• NCBI Protein Information: interleukin-34
• UniProt Protein Name: Interleukin-34
• UniProt Gene Name: IL34
• UniProt Synonym Gene Names: C16orf77; IL-34
• UniProt Entry Name: IL34_HUMAN

NCBI Description

Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]

Uniprot Description

IL34: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Belongs to the IL-34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cytokine; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of cell proliferation; innate immune response; positive regulation of protein amino acid phosphorylation; inflammatory response