Typical Testing Data/Standard Curve (for reference only)
Mouse CCL8/MCP2 ELISA Kit | CCL8 elisa kit
Mouse CCL8/MCP2 PicoKine ELISA Kit
Reactivity
Mouse
Synonyms
CCL8/MCP2; Mouse CCL8/MCP2 PicoKine ELISA Kit; C-C motif chemokine 8; chemokine (C-C motif) ligand 8; C C motif chemokine 8; CCL 8; Ccl8; CCL8_HUMAN; Chemokine (C C motif) ligand 8; Chemokine ligand 8; HC14; MCP 2; MCP-2; MCP-2(6-76); Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; SCYA10; SCYA8; Small inducible cytokine A8; Small inducible cytokine subfamily A (Cys Cys) member 8; Small-inducible cytokine A8; CCL8 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse CCL5
Sequence Length
97
Samples
Cell Culture Supernates. Serum And Plasma(Heparin, Edta, Citrate)
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCL8 elisa kit
Principle of the Assay: The Picokine Mouse CCL5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse CCL5 with a 96-well strip plate that is pre-coated with antibody specific for CCL5. The detection antibody is a biotinylated antibody specific for CCL5. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse CCL5 with immunogen: Expression system for standard: E.coli; Immunogen sequence: S24-S91. The kit is analytically validated with ready to use reagents. To measure Mouse CCL5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse CCL5 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse CCL5 in the sample!!Background/Introduction: Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
11,017 Da
NCBI Official Full Name
C-C motif chemokine 8
NCBI Official Symbol
LOC102641794
NCBI Protein Information
c-C motif chemokine 8-like
UniProt Protein Name
C-C motif chemokine 8
Protein Family
UniProt Gene Name
Ccl8
UniProt Synonym Gene Names
Mcp2; Scya8; MCP-2
UniProt Entry Name
CCL8_MOUSE
Uniprot Description
CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; chemokine activity; cytokine activity
Biological Process: immune response; inflammatory response; chemotaxis; positive regulation of myoblast differentiation
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Product Notes
The Mouse CCL8 ccl8 (Catalog #AAA177281) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177281 ELISA Kit recognizes Mouse CCL8. It is sometimes possible for the material contained within the vial of "CCL8/MCP2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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