Western Blot (WB)
(Western blotting analysis of isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli. Immunostained by anti-GST (S-tag-05).Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-SepharoseLane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02))
Mouse GST tag Antibody | anti-GST antibody
Anti-GST tag Purified
Reactivity
Tagged fusion proteins in all species
Applications
Western Blot, Immunoprecipitation
Purity
Purified by Protein-A Affinity Chromatography
Synonyms
GST tag; Anti-GST tag Purified; Glutathione-S-Transferase; Microsomal GST-1; anti-GST antibody
Host
Mouse
Reactivity
Tagged fusion proteins in all species
Isotype
IgG2b
Clone Number
S-tag-05
Specificity
The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibod is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-GST antibody
Western Blot (WB), Immunoprecipitation (IP)
Immunogen
GST fusion protein
Preparation and Storage
Store at 2-8 degree C.
Western Blot (WB)
(Western blotting analysis of isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli. Immunostained by anti-GST (S-tag-05).Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-SepharoseLane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02))
Western Blot (WB)
(Western blotting analysis of isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli. Immunostained by anti-GST (S-tag-05).Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-SepharoseLane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02))
Related Product Information for anti-GST antibody
The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.
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Product Notes
The GST (Catalog #AAA1801556) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-GST tag Purified reacts with Tagged fusion proteins in all species and may cross-react with other species as described in the data sheet. AAA Biotech's GST tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the GST for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GST tag, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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