Testing Data
Mouse GST Monoclonal Antibody | anti-GST antibody
Anti-GST Antibody
Applications
Western Blot, ELISA, Immunoprecipitation
Synonyms
GST; Monoclonal Antibody; Anti-GST Antibody; anti-GST antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
GST.B6
Specificity
Detects overexpressed glutathione-transferase (GST) fusion proteins. Recognizes native and denatured forms of purified GST or GST fusion proteins
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
208
Applicable Applications for anti-GST antibody
Western Blot (WB), IS, ELISA (EIA), Immunoprecipitation (IP)
Application Notes
Western blot (1:1000)
Immunoprecipitation and Immunostaining (1:200).
Immunoprecipitation and Immunostaining (1:200).
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Immunogen
Anti-GST antibody has been raised against whole GST protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-GST antibody
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
References
Xue, ZH et al. "A role of kindlin-3 in integrin alphaMbeta2 outside-in signaling and the Syk-Vav1-Rac1/Cdc42 signaling axis." PLoS One 8 (2):356911 (2013). DOI: 10.1371/journal.pone.0056911. Application: Reporter of siRNA transduction system.
Ding, Y et al. "ATX1-generated H3K4me3 is required for efficient elongation of transcription, not initiation, at ATX1-regulated genes." PLoS Genet. 8:e1003111 (2012). PubMed: 23284292.
Ding, Y et al. "Two distinct roles of ARABIDOPSIS HOMOLOG OF TRITHORAX1 (ATX1) at promoters and within transcribed regions of ATX1-regulated genes." Plant Cell 23 (1):350-363 (2011). DOI: 10.1105/tpc.110.080150. Application: Immunoblot.
Son, O et al. "In vitro and in vivo interaction of AtRma2 E3 ubiquitin ligase and auxin binding protein 1." Biochem Biophys Res Commun 393 (3):492-497 (2010). DOI: 10.1016/j.bbrc.2010.02.032. Application: Immunoblot.
Gusarova, GA et al. "Alpha1-AMP-activated protein kinase regulates hypoxia-induced Na,K-ATPase endocytosis via direct phosphorylation of protein kinase C zeta." Mol Cell Biol 13:3455-3464 (2009). DOI: 10.1128/MCB.00054-09. Application: Western Blot.
Xue, Y et al. "FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair." Hum. Mol. Genet. 17:1641-52 (2008). PubMed: 18285517.
Ding, Y et al. "ATX1-generated H3K4me3 is required for efficient elongation of transcription, not initiation, at ATX1-regulated genes." PLoS Genet. 8:e1003111 (2012). PubMed: 23284292.
Ding, Y et al. "Two distinct roles of ARABIDOPSIS HOMOLOG OF TRITHORAX1 (ATX1) at promoters and within transcribed regions of ATX1-regulated genes." Plant Cell 23 (1):350-363 (2011). DOI: 10.1105/tpc.110.080150. Application: Immunoblot.
Son, O et al. "In vitro and in vivo interaction of AtRma2 E3 ubiquitin ligase and auxin binding protein 1." Biochem Biophys Res Commun 393 (3):492-497 (2010). DOI: 10.1016/j.bbrc.2010.02.032. Application: Immunoblot.
Gusarova, GA et al. "Alpha1-AMP-activated protein kinase regulates hypoxia-induced Na,K-ATPase endocytosis via direct phosphorylation of protein kinase C zeta." Mol Cell Biol 13:3455-3464 (2009). DOI: 10.1128/MCB.00054-09. Application: Western Blot.
Xue, Y et al. "FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair." Hum. Mol. Genet. 17:1641-52 (2008). PubMed: 18285517.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
23,663 Da
NCBI Official Full Name
gst
UniProt Protein Name
Gst protein
Protein Family
UniProt Gene Name
gst
UniProt Entry Name
Q6MHF5_BDEBA
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Product Notes
The GST gst (Catalog #AAA415294) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IS, ELISA (EIA), Immunoprecipitation (IP). Western blot (1:1000) Immunoprecipitation and Immunostaining (1:200). Researchers should empirically determine the suitability of the GST gst for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GST, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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