Western Blot (WB)
(Western blot analysis of lysate from 12 tag recombinant protein, using GST Tag Antibody. MBS9200043 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.)
Mouse GST Tag Monoclonal Antibody
GST Tag Antibody
Applications
Western Blot, ELISA
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
GST Tag; Monoclonal Antibody; GST Tag Antibody; Glutathione S-transferase; anti-GST Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
9AT106
Specificity
Purified recombinant GST fusion protein was used to produced this monoclonal antibody.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Applicable Applications for anti-GST Tag antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2,000
Crown Antibody
Yes
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysate from 12 tag recombinant protein, using GST Tag Antibody. MBS9200043 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(Western blot analysis of lysate from 12 tag recombinant protein, using GST Tag Antibody. MBS9200043 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB)
(The anti-GST Mab is used in Western blot to detect GST recombinant protein purified from bacterial lysate.)
Western Blot (WB)
(The anti-GST Mab is used in Western blot to detect GST recombinant protein purified from bacterial lysate.)
Related Product Information for anti-GST Tag antibody
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner.
Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the MyBioSource anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the MyBioSource anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Product Categories/Family for anti-GST Tag antibody
References
Smith, D.B. and Johnson, K.S., (1988). Gene 67, 31.
Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221.
Toye, B. et al., (1990) Infect. Immun. 58, 3909.
Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262
Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221.
Toye, B. et al., (1990) Infect. Immun. 58, 3909.
Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262
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Product Notes
The GST Tag (Catalog #AAA9200043) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GST Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2,000. Researchers should empirically determine the suitability of the GST Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GST Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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