Testing Data
(Image: Cholecystokinin 8 terminals and fibers in rat dorsal horn (spinal cord), vibratome sections, ABC detection.)
Rabbit anti-Mouse, Rat CCK-8 Antibody | anti-CCK-8 antibody
CCK-8 (Cholecystokinin Octapeptide) Antibody
Reactivity
Mouse, Rat
Applications
Immunofluorescence
Synonyms
CCK-8; CCK-8 (Cholecystokinin Octapeptide) Antibody; anti-CCK-8 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Form/Format
Lyophilized 100 ul with 0.09% sodium azdie as a preservative.
Sequence Length
33
Applicable Applications for anti-CCK-8 antibody
Immunofluorescence (IF)
Application Notes
Immunofluroescence: 1:100-1:200 (Fluorescein) 1:500-1:1,000 (Streptavidin/HRP).
Dilutions listed as a recommendation.
Tissue Preparation:
10 um cryostats
•Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30 min.
•Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
Immunohistochemistry:
The antibody has siginificant indirect immunofluorescent staining at a 1/100-1/200 dilution and significant Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1,000 dilution in rat hypothalamus and spinal cord. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-6M CCK-8 immunolabeling was completely abolished by pre-adsorption with CCK-8, gastrin 17 and gastrin 34. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: alphaACGRP, BETACGRP, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, neuropeptide Y, gastric inhibitory polypeptide, bombesin, glucagon, peptide YY, and FMRF amide.
Optimal dilution should be determined by investigator.
Dilutions listed as a recommendation.
Tissue Preparation:
10 um cryostats
•Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30 min.
•Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
Immunohistochemistry:
The antibody has siginificant indirect immunofluorescent staining at a 1/100-1/200 dilution and significant Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1,000 dilution in rat hypothalamus and spinal cord. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-6M CCK-8 immunolabeling was completely abolished by pre-adsorption with CCK-8, gastrin 17 and gastrin 34. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: alphaACGRP, BETACGRP, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, neuropeptide Y, gastric inhibitory polypeptide, bombesin, glucagon, peptide YY, and FMRF amide.
Optimal dilution should be determined by investigator.
Immunogen Sequence
Sulfated CCK-8 (26-33) coupled to bovine thyroglobulin (BTg) with glutaraldehyde.
Reconstitution
Do not reconstitute until ready to use since the product is most stable when lyophilized. The product does not need to be cooled during shipping. For long-term storage, store lyophilized antibody until ready to use at -15º C or lower. Reconstitute with 100 ?L of distilled or deionized water.
Preparation and Storage
Maintain at 2-8 C for 3 months or at -20 degree C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.
Testing Data
(Image: Cholecystokinin 8 terminals and fibers in rat dorsal horn (spinal cord), vibratome sections, ABC detection.)
Testing Data
(Image: Cholecystokinin 8 terminals and fibers in rat dorsal horn (spinal cord), vibratome sections, ABC detection.)
Similar Products
Product Notes
The CCK-8 (Catalog #AAA555736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCK-8 (Cholecystokinin Octapeptide) Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CCK-8 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). Immunofluroescence: 1:100-1:200 (Fluorescein) 1:500-1:1,000 (Streptavidin/HRP). Dilutions listed as a recommendation. Tissue Preparation: 10 um cryostats •Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30 min. •Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Immunohistochemistry: The antibody has siginificant indirect immunofluorescent staining at a 1/100-1/200 dilution and significant Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1,000 dilution in rat hypothalamus and spinal cord. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-6M CCK-8 immunolabeling was completely abolished by pre-adsorption with CCK-8, gastrin 17 and gastrin 34. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: alphaACGRP, BETACGRP, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, neuropeptide Y, gastric inhibitory polypeptide, bombesin, glucagon, peptide YY, and FMRF amide. Optimal dilution should be determined by investigator. Researchers should empirically determine the suitability of the CCK-8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCK-8, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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