Mouse CD74 Antibody | anti-CD74 antibody
Mouse Anti-Human CD74-PE
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Immunoblot, Western Blot
Synonyms
CD74; Mouse Anti-Human CD74-PE; Invariant chain; Ii; class II-specific chaperone; anti-CD74 antibody
Host
Mouse
Isotype
IgG1kappa
Clone Number
LN2
Specificity
Human CD74
Sequence Length
214
Applicable Applications for anti-CD74 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin/ Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunoblot (IB), Blocking (BL)
Application Notes
Flow Cytometry - Quality tested; Immunohistochemistry-Frozen Sections - Reported in literature; Immunohistochemistry-Paraffin Sections - Reported in literature; Immunocytochemistry - Reported in literature; Immunoprecipitation - Reported in literature; Immunoblotting - Reported in literature; Blocking - Reported in literature
Immunogen
Cell line SU-DHL-4 nuclei
Buffer Formulation
Supplied in PBS/NaN3 and a stabilizing agent
Isotype Control
Mouse IgG1-PE
Preparation and Storage
Store at 2-8 degree C
Related Product Information for anti-CD74 antibody
CD74 represents a type II integral membrane protein known as MHC class II-associated invariant chain. It is moderately expressed on B cells and weakly on monocytes and macrophages. CD74 is also found on a subset of activated T cells, Langerhans cells, dendritic cells, and some epithelial cells. CD74 may play a role in antigen processing and presentation through intracellular transport and expression of class II molecules on the cell surface.
References
1. Epstein AL, Marder RJ, Winter JN, Fox RI. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984;133:1028-36. (Immunogen, IHC-FS, IHC-PS, ICC, IP)
2. Pack M, Trumpfheller C, Thomas D, Park CG, Granelli-Piperno A, Münz C, et al. DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp. Immunology. 2007;123:438-46. (IHC-FS)
3. Sarker AB, Akagi T, Jeon HJ, Miyake K, Murakami I, Yoshino T, et al. Bauhinia purpurea--a new paraffin section marker for Reed-Sternberg cells of Hodgkin's disease. A comparison with Leu-M1 (CD15), LN2 (CD74), peanut agglutinin, and Ber-H2 (CD30). Am J Pathol. 1992;141:19-23. (IHC-PS)
4. Burton JD, Ely S, Reddy PK, Stein R, Gold DV, Cardillo TM, et al. CD74 is expressed by multiple myeloma and is a promising target for therapy. Clin Cancer Res. 2004;10:6606-11. (IHC-PS, FC)
5. Sepp N, Radaszkiewicz T, Meijer CJ, Smolle J, Seewann H, Fritsch P, et al. Specific skin manifestations in acute leukemia with monocytic differentiation. A morphologic and immunohistochemical study of 11 cases. Cancer. 1993;71:124-32. (IHC-PS)
6. Triantafilou K, Triantafilou M, Wilson KM, Cherry RJ, Fernandez N. Intracellular and cell surface heterotypic associations of human leukocyte antigen-DR and human invariant chain. Hum Immunol. 1999;60:1101-12. (ICC, IP, FC)
7. Imam A, Taylor CR, inventors; University of Southern California, assignee. Antigen specifically expressed on the surface of B cells and Hodgkin's cells. United States patent US 5304635. 1994 Apr 19. (WB)
8. Zhou C, Lu L, Tan S, Jiang S, Chen Y. HIV-1 glycoprotein 41 ectodomain induces activation of the CD74 protein-mediated extracellular signal-regulated kinase/mitogen-activated protein kinase pathway to enhance viral infection. J Biol Chem. 2011;286:44869-77. (Block)
9. Klasen C, Ohl K, Sternkopf M, Schachar I, Schmitz C, Heussen N, et al. MIF promotes B cell chemotaxis through the receptors CXCR4 and CD74 and ZAP-70 signaling. J Immunol. 2014;192:5273-84. (Block)
10. Aiello A, Delia D, Borrello MG, Biassoni D, Giardini R, Fontanella E, et al. Flow cytometric detection of the mitochondrial BCL-2 protein in normal and neoplastic human lymphoid cells. Cytometry. 1992;13:502-9. (FC)
11. Butrym A, Majewski M, Dzietczenia J, Kuliczkowski K, Mazur G. High CD74 expression correlates with ZAP70 expression in B cell chronic lymphocytic leukemia patients. Med Oncol. 2013;30:560. (FC)
2. Pack M, Trumpfheller C, Thomas D, Park CG, Granelli-Piperno A, Münz C, et al. DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp. Immunology. 2007;123:438-46. (IHC-FS)
3. Sarker AB, Akagi T, Jeon HJ, Miyake K, Murakami I, Yoshino T, et al. Bauhinia purpurea--a new paraffin section marker for Reed-Sternberg cells of Hodgkin's disease. A comparison with Leu-M1 (CD15), LN2 (CD74), peanut agglutinin, and Ber-H2 (CD30). Am J Pathol. 1992;141:19-23. (IHC-PS)
4. Burton JD, Ely S, Reddy PK, Stein R, Gold DV, Cardillo TM, et al. CD74 is expressed by multiple myeloma and is a promising target for therapy. Clin Cancer Res. 2004;10:6606-11. (IHC-PS, FC)
5. Sepp N, Radaszkiewicz T, Meijer CJ, Smolle J, Seewann H, Fritsch P, et al. Specific skin manifestations in acute leukemia with monocytic differentiation. A morphologic and immunohistochemical study of 11 cases. Cancer. 1993;71:124-32. (IHC-PS)
6. Triantafilou K, Triantafilou M, Wilson KM, Cherry RJ, Fernandez N. Intracellular and cell surface heterotypic associations of human leukocyte antigen-DR and human invariant chain. Hum Immunol. 1999;60:1101-12. (ICC, IP, FC)
7. Imam A, Taylor CR, inventors; University of Southern California, assignee. Antigen specifically expressed on the surface of B cells and Hodgkin's cells. United States patent US 5304635. 1994 Apr 19. (WB)
8. Zhou C, Lu L, Tan S, Jiang S, Chen Y. HIV-1 glycoprotein 41 ectodomain induces activation of the CD74 protein-mediated extracellular signal-regulated kinase/mitogen-activated protein kinase pathway to enhance viral infection. J Biol Chem. 2011;286:44869-77. (Block)
9. Klasen C, Ohl K, Sternkopf M, Schachar I, Schmitz C, Heussen N, et al. MIF promotes B cell chemotaxis through the receptors CXCR4 and CD74 and ZAP-70 signaling. J Immunol. 2014;192:5273-84. (Block)
10. Aiello A, Delia D, Borrello MG, Biassoni D, Giardini R, Fontanella E, et al. Flow cytometric detection of the mitochondrial BCL-2 protein in normal and neoplastic human lymphoid cells. Cytometry. 1992;13:502-9. (FC)
11. Butrym A, Majewski M, Dzietczenia J, Kuliczkowski K, Mazur G. High CD74 expression correlates with ZAP70 expression in B cell chronic lymphocytic leukemia patients. Med Oncol. 2013;30:560. (FC)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD74 antigen
NCBI Official Symbol
CD74
NCBI Protein Information
CD74 antigen
Protein Family
Research Articles on CD74
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Product Notes
The CD74 (Catalog #AAA670612) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD74 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin/ Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunoblot (IB), Blocking (BL). Flow Cytometry - Quality tested; Immunohistochemistry-Frozen Sections - Reported in literature; Immunohistochemistry-Paraffin Sections - Reported in literature; Immunocytochemistry - Reported in literature; Immunoprecipitation - Reported in literature; Immunoblotting - Reported in literature; Blocking - Reported in literature. Researchers should empirically determine the suitability of the CD74 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD74, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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