Mouse CD74 Monoclonal Antibody | anti-CD74 antibody

Anti-CD74 Antibody (B-Cell Marker) (Clone LN-2)

Cat. Num. AAA847420

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human, Baboon, Mouse; Does not react with rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Protein A/G purification
• Synonyms: CD74; Monoclonal Antibody; Anti-CD74 Antibody (B-Cell Marker) (Clone LN-2); DHLAG; Gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLADR-gamma (HLADG); Ia antigen-associated invariant chain; la-gamma; Major histocompatibility complex class II invariant chain; CLIP; MHC HLA-DR gamma chain; p33; anti-CD74 antibody

• Host: Mouse
• Reactivity: Human, Baboon, Mouse; Does not react with rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone Number: LN-2
• Specificity: Monoclonal Antibody to detect CD74 in human samples
• Purity/Purification: Protein A/G purification
• Form/Format: Colorless liquid. In 10mM PBS with 0.05% BSA & 0.05% azide

• Applicable Applications for anti-CD74 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: FC/FACS: 0.5-1ug/1x106 cells in 0.1 ml; IF: 1-2ug/ml; IHC: 1-2ug/ml for 30 minutes at room temperature
• Immunogen: SU-DHL-4 lymphoma cells
• Positive Control: IHC: Human Tonsil
• Preparation and Storage: Store at -20 degree C; The antibody solution should be gently mixed before use; Shelf life: ~12 months

Immunohistochemistry (IHC)

Related Product Information for anti-CD74 antibody

It recognizes a protein of ~35 kDa, identified as CD74. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating a typical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .

Product Categories/Family for anti-CD74 antibody

Antibodies & Supporting Tools; Marker Antibodies; Membrane

NCBI and Uniprot Product Information

• NCBI GI #: 20178292
• NCBI GeneID: 972
• UniProt Accession #: P04233; Q14597; Q29832; Q5U0J8; Q8SNA0; Q8WLP6; A8K7R1; B4DNE8; D3DQG3; D3DQG4
• Molecular Weight: 296
• NCBI Official Full Name: HLA class II histocompatibility antigen gamma chain
• NCBI Official Synonym Full Names: CD74 molecule, major histocompatibility complex, class II invariant chain
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histo
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii
• UniProt Entry Name: HG2A_HUMAN

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CD74: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74- ROS1 chimeric protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: lysosomal lumen; cell surface; lysosomal membrane; integral to membrane; MHC class II protein complex; Golgi membrane; multivesicular body; membrane; plasma membrane; vacuole; trans-Golgi network membrane; intracellular; external side of plasma membrane

Molecular Function: identical protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; beta-amyloid binding; cytokine binding; MHC class II protein binding, via antigen binding groove; nitric-oxide synthase binding; MHC class II protein binding

Biological Process: activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class II; defense response; prostaglandin biosynthetic process; signal transduction; negative regulation of T cell differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; intracellular protein transport; positive regulation of fibroblast proliferation; negative regulation of mature B cell apoptosis; positive regulation of B cell proliferation; protein complex assembly; positive thymic T cell selection; negative regulation of peptide secretion; positive regulation of T-helper 2 type immune response; chaperone cofactor-dependent protein folding; regulation of macrophage activation; T cell selection; negative thymic T cell selection; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell differentiation; immunoglobulin mediated immune response; positive regulation of cytokine and chemokine mediated signaling pathway; antigen processing and presentation of endogenous antigen; negative regulation of apoptosis