Human Ubiquilin 2 (UBQLN2) RTU ELISA Kit | UBQLN2 rtu elisa kit

Human Ubiquilin 2 (UBQLN2) ELISA Kit

Cat. Num. AAA4503188

• Gene Names: UBQLN2; DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157
• Reactivity: Human
• Synonyms: Ubiquilin 2 (UBQLN2); Human Ubiquilin 2 (UBQLN2) ELISA Kit; CHAP1; CHAP1/DSK2; Dsk2; LIC-2; N4BP4; PLIC-2; PLIC2; NEDD4 Binding Protein 4; Protein linking IAP with cytoskeleton 2; Ubiquitin-like product Chap1/Dsk2; UBQLN2 rtu elisa kit

• Reactivity: Human
• Sequence Length: 624

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.64ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UBQLN2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to UBQLN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to UBQLN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UBQLN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UBQLN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16753207
• NCBI GeneID: 29978
• NCBI Accession #: NP_038472.2
• NCBI GenBank Nucleotide #: NM_013444.3
• UniProt Accession #: Q9UHD9; O94798; Q5D027; Q9H3W6; Q9HAZ4
• Molecular Weight: 65,696 Da
• NCBI Official Full Name: ubiquilin-2
• NCBI Official Synonym Full Names: ubiquilin 2
• NCBI Official Symbol: UBQLN2
• NCBI Official Synonym Symbols: DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157
• NCBI Protein Information: ubiquilin-2
• UniProt Protein Name: Ubiquilin-2
• Protein Family: Ubiquilin
• UniProt Gene Name: UBQLN2
• UniProt Synonym Gene Names: N4BP4; PLIC2; PLIC-2; hPLIC-2

NCBI Description

This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein. [provided by RefSeq, Oct 2009]

Uniprot Description

Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed:10983987). Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome (PubMed:24215460, PubMed:18307982). Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:19148225, PubMed:20529957). Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits (CCPs) (PubMed:18199683).