Rabbit S1P1 Polyclonal Antibody | anti-S1P1 antibody

S1P1 Antibody

Cat. Num. AAA9410370

• Gene Names: S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: S1P1; Polyclonal Antibody; S1P1 Antibody; Sphingosine-1-phosphate receptor 1; S1PR1; EDG1; ECGF1; anti-S1P1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: At least two isoforms of S1P1 are known to exist; this S1P1 antibody will only recognize the shorter isoform.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 382

• Applicable Applications for anti-S1P1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 14 amino acid peptide near the carboxy terminus of the human S1P1.
• Target Name: S1P1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of S1P1 in mouse thymus lysate with S1P1 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of S1P1 in mouse thymus tissue with S1P1 antibody at 5 ug/mL.)

Related Product Information for anti-S1P1 antibody

Movement of lymphocytes through lymphoid organs is required for generating immunity. Their migration into lymph nodes follows a series of events including integrin activation through chemokine signaling, adhesion and diapedis. The release of lymphocytes from lymph nodes is regulated by the phospholipid sphingosine-1-phosphate (S1P). One of its receptors S1P1 binds S1P with high specificity and affinity; agonism of this receptor by the immunosuppressive agent FTY720 inhibits the entry of tissue T cells into afferent lymphatics in homeostatic and inflammatory conditions. Recent experiments have indicated that CCR7-deficient T cells left lymph nodes more rapidly than wild-type cells did and these cells where also less effectively retained after treatment with FTY720, and that egress competence could be restored by inactivating G alpha i-protein-coupled receptor signaling. These results suggest that S1P1 acts in the lymphocyte to promote lymph node egress by overcoming retention signals mediated by CCR7 and G alpha i-protein-coupled receptor signaling.

Product Categories/Family for anti-S1P1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 13027636
• NCBI GeneID: 1901
• NCBI Accession #: NP_001391
• NCBI GenBank Nucleotide #: NM_001400.4
• NCBI Official Full Name: sphingosine 1-phosphate receptor 1
• NCBI Official Synonym Full Names: sphingosine-1-phosphate receptor 1
• NCBI Official Symbol: S1PR1
• NCBI Official Synonym Symbols: EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
• NCBI Protein Information: sphingosine 1-phosphate receptor 1
• UniProt Protein Name: Sphingosine 1-phosphate receptor 1
• UniProt Gene Name: S1PR1
• UniProt Synonym Gene Names: CHEDG1; EDG1; S1P receptor 1; S1P1; S1P receptor Edg-1
• UniProt Entry Name: S1PR1_HUMAN

NCBI Description

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq, Jul 2008]

Uniprot Description

EDG-1: a G protein-coupled receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Highly expressed in endothelial cells and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Suggested to be involved in the processes that regulate the migration/differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. S1P-induced endothelial cell migration requires AKT1-mediated phosphorylation of the third intracellular loop. Seems to be coupled to the G(i) subclass of heteromeric G proteins.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: integral to membrane; plasma membrane; endosome; intrinsic to plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; sphingolipid binding; G-protein-coupled receptor binding

Biological Process: regulation of cell adhesion; lamellipodium biogenesis; endothelial cell differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of bone resorption; cell migration; positive regulation of positive chemotaxis; negative regulation of stress fiber formation; positive regulation of smooth muscle cell proliferation; transmission of nerve impulse; chemotaxis; blood vessel maturation; neuron differentiation; G-protein coupled receptor protein signaling pathway; actin cytoskeleton reorganization; positive regulation of transcription from RNA polymerase II promoter; brain development; G-protein signaling, adenylate cyclase inhibiting pathway; angiogenesis; cell adhesion; regulation of bone mineralization; positive regulation of cell migration