Mouse anti-Human ZAP-70 (TCR zeta) Monoclonal Antibody | anti-Zap70 antibody

Mouse Anti-Human ZAP-70-UNLB

Cat. Num. AAA670105

• Gene Names: Zap70; Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364
• Reactivity: Human
• Applications: ELISA, Flow Cytometry
• Synonyms: ZAP-70 (TCR zeta); Monoclonal Antibody; Mouse Anti-Human ZAP-70-UNLB; Mouse Anti-Human ZAP-70 - Purified; Unlabeled Antibody; Mouse Anti-Human ZAP-70 (TCR zeta); anti-Zap70 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: SBZAP
• Specificity: Human TCR zeta ; Zap-70 (Mr 70 kDa); ZAP 70, also known as TCR zeta associated protein, is a protein tyrosine kinase (PTK) with the primary role of catalyzing the phosphorylation of tyrosine residues in cellular proteins, and thus, contributes significantly to the initiation of cell signaling. ZAP 70 is expressed on T cells and NK cells. The monoclonal antibodies SB70 and SBZAP were generated against a KLH-peptide sequence corresponding to the human ZAP 70 amino acid residues 280-309.
• Form/Format: Purified (UNLB) Antibody

• Applicable Applications for anti-Zap70 antibody: ELISA; Flow Cytometry
• Application Notes: Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
• Antigen Description: Human ZAP-70 amino acid residues 280-309
• Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or PCFIA assay to conform to the characteristics of a standard reference reagent.
• Preparation and Storage: The purified antibody is supplied as 0.025 mg purified immunoglobulin in 0.25 mL or 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at - 20 degree C. ; The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. ; The biotin (BIOT) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL in PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

References

1. Wange, R.L., N. Isakov, T.R. Burke., A. Otaka, P.P. Roller, J.D. Watts, R. Aebersold, and L.E. Samelson. 1995. J. Biol. Chem. 270:944.
3. Van Oers, N.S., and A. Weiss. 1995. Semin. Immunol. 7:227.

NCBI and Uniprot Product Information

• NCBI GI #: 436480
• NCBI GeneID: 22637
• UniProt Accession #: P43404; P97455; Q80VV2; Q8CHJ3
• Molecular Weight: 70,058 Da
• NCBI Official Full Name: ZAP-70
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase
• NCBI Official Symbol: Zap70
• NCBI Official Synonym Symbols: Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364
• NCBI Protein Information: tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Zap70
• UniProt Synonym Gene Names: Srk; Zap-70
• UniProt Entry Name: ZAP70_MOUSE

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Function: Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. Ref.3

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Interacts with CBL. Interacts with SHB. Interacts with FCRL3

By similarity. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with SLA and SLA2 when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with CBLB and DEF6. Interacts (via SH2 domains) with RHOH. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location: Cytoplasm. Cell membrane. Note: After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. Ref.3 Ref.10

Tissue specificity: Isoform 1 and isoform 2 are expressed in thymus, sleen and lymph nodes. Ref.3

Developmental stage: Isoform 2 is expressed in develoing thymocytes from the CD44+CD25- stage up to mature T cells. Isoform 1 is not expressed in thymocytes at the CD44+CD25- or CD44+CD25+ stages. Ref.3

Domain: The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. Ref.10

Post-translational modification: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-318 phosphorylation is essential for full activity

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.Contains 1 protein kinase domain.Contains 2 SH2 domains.