Rat F4/80 Monoclonal Antibody | anti-F4/80 antibody

RAT ANTI MOUSE F4/80

Cat. Num. AAA216417

• Gene Names: Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, immunoassay, Radioimmunoassay, Immunohistochemistry, Western Blot
• Synonyms: F4/80; Monoclonal Antibody; RAT ANTI MOUSE F4/80; anti-F4/80 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: Cl:A3-1
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 931

• Applicable Applications for anti-F4/80 antibody: Immunohistology Frozen, Immunoelectron Microscopy (EM), Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Radioimmunoassays (RIA), Immunohistology Resin (RE), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Immunohistology - Paraffin: Application Note: F4/80 antibody requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours.
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC (MBS216414))

Testing Data

(Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 (MBS216424), red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Testing Data

(Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC (MBS216408))

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750 (MBS219371P750))

Testing Data

(Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin (MBS216412))

Testing Data

(Frozen mouse spleen stained with A: Rat anti Mouse F4/80 (MBS219371) followed by Goat anti Rat IgG:HRP (MBS235195) or B: Rat anti Mouse F4/80 (MBS219371) preincubated with 2 molar excess of Human anti Idiotypic MBS219371 (HCA154) followed by Goat anti Rat IgG:HRP (MBS235195))

Testing Data

(Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin (MBS216409))

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue(MBS219371PB))

Testing Data

(Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Testing Data

(Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin (MBS216410))

NCBI and Uniprot Product Information

• NCBI GI #: 33859546
• NCBI GeneID: 13733
• NCBI Accession #: NP_034260.1
• NCBI GenBank Nucleotide #: NM_010130.4
• Molecular Weight: 102,130 Da
• NCBI Official Full Name: EGF-like module-containing mucin-like hormone receptor-like 1
• NCBI Official Synonym Full Names: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• NCBI Official Symbol: Emr1
• NCBI Official Synonym Symbols: Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
• NCBI Protein Information: EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71
• UniProt Protein Name: EGF-like module-containing mucin-like hormone receptor-like 1
• UniProt Gene Name: Emr1
• UniProt Synonym Gene Names: Gpf480
• UniProt Entry Name: EMR1_MOUSE

Uniprot Description

EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 2; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Cellular Component: membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction