Mouse anti-Human X-prolyl aminopeptidase Monoclonal Antibody | anti-Xpnpep1 antibody
X-prolyl aminopeptidase (aminopeptidase P, 2 Aminoacylproline aminopeptidase, mAmP, Membrane-bound aminopeptidase P, Membrane-bound AmP, Membrane-bound APP, Xaa-Pro aminopeptidase 2 precursor, X-Pro aminopeptidase 2)
Gene Names
Xpnpep1; D230045I08Rik; Xpnpep1
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation, Neutralization Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
X-prolyl aminopeptidase; Monoclonal Antibody; X-prolyl aminopeptidase (aminopeptidase P; 2 Aminoacylproline aminopeptidase; mAmP; Membrane-bound aminopeptidase P; Membrane-bound AmP; Membrane-bound APP; Xaa-Pro aminopeptidase 2 precursor; X-Pro aminopeptidase 2); Anti -X-prolyl aminopeptidase (aminopeptidase P; anti-Xpnpep1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8B54
Specificity
Recognizes human X-prolyl aminopeptidase. Does not crossreact with recombinant human XPNEP-1 or recombinant mouse XNEP-2. Species Sequence Homology: chimpanzee; 95%, canine; 84%, rat; 82%, mouse; 81%.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Applicable Applications for anti-Xpnpep1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Neutralization
Application Notes
Suitable for use in Western Blot, Immunoprecipitation, ELISA and Neutralization.
Dilution: Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~10ng/lane under non-reducing and reducing conditions.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human X-prolyl aminopeptidase from conditioned cell medium.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well.
Neutralization: Inhibits/neutralizes the enzymatic activity of recombinant human X-prolyl aminopeptidase. X1049-56A was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with K(Abz)PP-pNA. 50% of the proteolytic activity was inhibited by X1049-56A at ~1.5ug/ml (IC50) under conditions in which the enzyme was present at 2ug/ml and the substrate concentration was 0.05mM. Considering the molecular masses of the enzyme (72kD) and the antibody (150kD), IC50 was achieved at ~1:2 molar ratio of the antibody to the enzyme.
Dilution: Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~10ng/lane under non-reducing and reducing conditions.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human X-prolyl aminopeptidase from conditioned cell medium.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well.
Neutralization: Inhibits/neutralizes the enzymatic activity of recombinant human X-prolyl aminopeptidase. X1049-56A was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with K(Abz)PP-pNA. 50% of the proteolytic activity was inhibited by X1049-56A at ~1.5ug/ml (IC50) under conditions in which the enzyme was present at 2ug/ml and the substrate concentration was 0.05mM. Considering the molecular masses of the enzyme (72kD) and the antibody (150kD), IC50 was achieved at ~1:2 molar ratio of the antibody to the enzyme.
Immunogen
NS0-derived recombinant human X-prolyl aminopeptidase (aa23-650, Accession #O43895).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Xpnpep1 antibody
Membrane-bound Aminopeptidase P2 is a hydrolase specific for N-terminal imido bonds. It is widely expressed in many adult tissues with the highest levels in the kidney.
Product Categories/Family for anti-Xpnpep1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
69,591 Da
NCBI Official Full Name
X-prolyl aminopeptidase
NCBI Official Synonym Full Names
X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
NCBI Official Symbol
Xpnpep1
NCBI Official Synonym Symbols
D230045I08Rik; Xpnpep1
NCBI Protein Information
xaa-Pro aminopeptidase 1; sAmp; X-Pro aminopeptidase 1; soluble aminopeptidase P; cytosolic aminopeptidase P; aminoacylproline aminopeptidase; X-prolyl aminopeptidase 1, soluble
UniProt Protein Name
X-prolyl aminopeptidase
UniProt Gene Name
Xpnpep1
UniProt Entry Name
Q3UKF5_MOUSE
Uniprot Description
XPNPEP1: Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. Belongs to the peptidase M24B family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.11.9; Protease
Cellular Component: cytoplasm; cytosol
Molecular Function: peptidase activity; protein homodimerization activity; hydrolase activity; metallopeptidase activity; manganese ion binding; metal ion binding; aminopeptidase activity
Biological Process: proteolysis
Similar Products
Product Notes
The Xpnpep1 xpnpep1 (Catalog #AAA600856) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The X-prolyl aminopeptidase (aminopeptidase P, 2 Aminoacylproline aminopeptidase, mAmP, Membrane-bound aminopeptidase P, Membrane-bound AmP, Membrane-bound APP, Xaa-Pro aminopeptidase 2 precursor, X-Pro aminopeptidase 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's X-prolyl aminopeptidase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Neutralization. Suitable for use in Western Blot, Immunoprecipitation, ELISA and Neutralization. Dilution: Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~10ng/lane under non-reducing and reducing conditions. Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human X-prolyl aminopeptidase from conditioned cell medium. Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well. Neutralization: Inhibits/neutralizes the enzymatic activity of recombinant human X-prolyl aminopeptidase. X1049-56A was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with K(Abz)PP-pNA. 50% of the proteolytic activity was inhibited by X1049-56A at ~1.5ug/ml (IC50) under conditions in which the enzyme was present at 2ug/ml and the substrate concentration was 0.05mM. Considering the molecular masses of the enzyme (72kD) and the antibody (150kD), IC50 was achieved at ~1:2 molar ratio of the antibody to the enzyme. Researchers should empirically determine the suitability of the Xpnpep1 xpnpep1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "X-prolyl aminopeptidase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
