XPNPEP2 active protein

XPNPEP2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121008

• Gene Names: XPNPEP2; APP2; AEACEI
• Purity: >97% as determined by SDS-PAGE
• Synonyms: XPNPEP2; XPNPEP2 Protein; Human; Recombinant (His Tag); Human XPNPEP2/X-Pro aminopeptidase 2 Protein (His Tag); AEACEI Protein; APP2 Protein; X-prolyl aminopeptidase (aminopeptidase P) 2; membrane-bound; XPNPEP2 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala650

• Species: Human
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate, H-Lys(2-Aminobenzoyl)Pro-Pro-pNitroanilide(K(Abz)PP-pNA). The specific activity is >300pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human XPNPEP2 (O43895)(Met 1-Ala 650) without the pro peptide was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for XPNPEP2 active protein

Background: Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds, which are common to several collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2, the X-linked gene that encodes membranous aminopeptidase P (APP), has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family, of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation, suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF).

References

Sprinkle TJ, et al. (2000) Cloning, chromosomal sublocalization of the human soluble aminopeptidase P gene (XPNPEP1) to 10q25.3 and conservation of the putative proton shuttle and metal ligand binding sites with XPNPEP2. Arch Biochem Biophys. 378(1): 51-6.Prueitt RL, et al. (2000) Physical mapping of nine Xq translocation breakpoints and identification of XPNPEP2 as a premature ovarian failure candidate gene. Cytogenet Cell Genet. 89(1-2): 44-50.Duan QL, et al. (2005) A variant in XPNPEP2 is associated with angioedema induced by angiotensin I-converting enzyme inhibitors. Am J Hum Genet. 77(4): 617-26.Woodard-Grice AV, et al. (2010) Sex-dependent and race-dependent association of XPNPEP2 C-2399A polymorphism with angiotensin-converting enzyme inhibitor-associated angioedema. Pharmacogenet Genomics. 20(9): 532-6.

NCBI and Uniprot Product Information

• NCBI GI #: 93141226
• NCBI GeneID: 7512
• NCBI Accession #: NP_003390.4
• NCBI GenBank Nucleotide #: NM_003399.5
• UniProt Accession #: O43895; O75994; A0AV16
• Molecular Weight: 75,625 Da
• NCBI Official Full Name: xaa-Pro aminopeptidase 2
• NCBI Official Synonym Full Names: X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound
• NCBI Official Symbol: XPNPEP2
• NCBI Official Synonym Symbols: APP2; AEACEI
• NCBI Protein Information: xaa-Pro aminopeptidase 2; mAmP; membrane-bound APP; membrane-bound AmP; X-Pro aminopeptidase 2; aminoacylproline aminopeptidase; membrane-bound aminopeptidase P
• UniProt Protein Name: Xaa-Pro aminopeptidase 2
• UniProt Gene Name: XPNPEP2
• UniProt Synonym Gene Names: Membrane-bound APP; Membrane-bound AmP; mAmP
• UniProt Entry Name: XPP2_HUMAN

NCBI Description

Aminopeptidase P is a hydrolase specific for N-terminal imido bonds, which are common to several collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. Structurally, the enzyme is a member of the 'pita bread fold' family and occurs in mammalian tissues in both soluble and GPI-anchored membrane-bound forms. A membrane-bound and soluble form of this enzyme have been identified as products of two separate genes. [provided by RefSeq, Jul 2008]

Uniprot Description

XPNPEP2: A metalloprotease that may play a role in the inflammatory process and other reactions produced in response to injury or infection. May also play a role in the metabolism of the vasodilator bradykinin. Belongs to the peptidase M24B family.

Protein type: Membrane protein, GPI anchor; Protease; EC 3.4.11.9

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: membrane; plasma membrane

Molecular Function: metallopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: proteolysis

Disease: Angioedema Induced By Ace Inhibitors, Susceptibility To