Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Epithelial-stromal interaction protein 1 (EPSTI1) Standard Curve.)
Human Epithelial-Stromal Interaction Protein 1 (EPSTI1) ELISA Kit | EPSTI1 elisa kit
Human Epithelial-Stromal Interaction Protein 1 (EPSTI1) ELISA Kit
Gene Names
EPSTI1; BRESI1
Reactivity
Human
Synonyms
Epithelial-Stromal Interaction Protein 1 (EPSTI1); Human Epithelial-Stromal Interaction Protein 1 (EPSTI1) ELISA Kit; EPSTI1; BRESI1; MGC29634; epithelial stromal interaction 1; EPSTI1 elisa kit
Reactivity
Human
Specificity
Human Epithelial-stromal interaction protein 1 (EPSTI1) ELISA Kit has high sensitivity and excellent specificity for detection of Human EPSTI1. No significant cross-reactivity or interference between Human EPSTI1 and analogues was observed.
Sequence Length
307
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Epithelial-stromal interaction protein 1 (EPSTI1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Epithelial-stromal interaction protein 1 (EPSTI1) Standard Curve.)
Related Product Information for EPSTI1 elisa kit
Description: This Human Epithelial-stromal interaction protein 1 (EPSTI1) ELISA Kit employs a two-site sandwich ELISA to quantitate EPSTI1 in samples. An antibody specific for EPSTI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPSTI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPSTI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPSTI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By differential display, Nielsen et al. (2002) identified EPSTI1 as a transcript that is induced by coculture of breast tumor cells and fibroblasts, and they generated the full-length cDNA by 5-prime RACE of normal breast or placenta RNA. The deduced 307-amino acid protein has a calculated molecular mass of about 35 kD. It contains no N-terminal signal sequence and no transmembrane domains; however, there are 3 coiled-coil regions. Northern blot analysis revealed a transcript size of 1.5 kb. Real-time PCR revealed highest expression in placenta; strong expression in small intestine, spleen, salivary gland, and testes; and low levels of expression in normal breast and several other tissues. The expression of EPSTI1 was upregulated up to 72 times (range 5.6-72.1) in all 14 breast carcinomas tested.
Background: By differential display, Nielsen et al. (2002) identified EPSTI1 as a transcript that is induced by coculture of breast tumor cells and fibroblasts, and they generated the full-length cDNA by 5-prime RACE of normal breast or placenta RNA. The deduced 307-amino acid protein has a calculated molecular mass of about 35 kD. It contains no N-terminal signal sequence and no transmembrane domains; however, there are 3 coiled-coil regions. Northern blot analysis revealed a transcript size of 1.5 kb. Real-time PCR revealed highest expression in placenta; strong expression in small intestine, spleen, salivary gland, and testes; and low levels of expression in normal breast and several other tissues. The expression of EPSTI1 was upregulated up to 72 times (range 5.6-72.1) in all 14 breast carcinomas tested.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
epithelial-stromal interaction protein 1 isoform 2
NCBI Official Synonym Full Names
epithelial stromal interaction 1
NCBI Official Symbol
EPSTI1
NCBI Official Synonym Symbols
BRESI1
NCBI Protein Information
epithelial-stromal interaction protein 1
UniProt Protein Name
Epithelial-stromal interaction protein 1
Protein Family
UniProt Gene Name
EPSTI1
UniProt Entry Name
ESIP1_HUMAN
NCBI Description
The protein encoded by this gene has been shown to promote tumor invasion and metastasis in some invasive cancer cells when overexpressed. Expression of this gene has been shown to be upregulated by direct binding of the Kruppel like factor 8 protein to promoter sequences. The translated protein interacts with the amino terminal region of the valosin containing protein gene product, resulting in the nuclear translocation of the nuclear factor kappa B subunit 1 gene product, and activation of target genes. Overexpression of this gene has been observed in some breast cancers and in some individuals with systemic lupus erythematosus (SLE). [provided by RefSeq, Sep 2016]
Uniprot Description
EPSTI1: 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 13q13.3
Research Articles on EPSTI1
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Product Notes
The Human EPSTI1 epsti1 (Catalog #AAA9716062) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716062 ELISA Kit recognizes Human EPSTI1. It is sometimes possible for the material contained within the vial of "Epithelial-Stromal Interaction Protein 1 (EPSTI1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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