Mouse anti-Human, Rat Vglut1 Monoclonal Antibody | anti-Vglut1 antibody

Vglut1 Mouse Monoclonal Antibody (McKA1)

Cat. Num. AAA588212

• Gene Names: SLC17A7; BNPI; VGLUT1
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, ELISA, Western Blot
• Synonyms: Vglut1; Monoclonal Antibody; Vglut1 Mouse Monoclonal Antibody (McKA1); Vesicular glutamate transporter 1; VGIuTI; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 7; anti-Vglut1 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: lgG2a
• Clone Number: McKA1
• Specificity: A highly specific and sensitive antibody for VGIutl. The antibody reveals the presence of the 58 kDa VGlut 1 protein on western blot of homogenized cerebral cortex cells lysates. It shows very precise reaction with VGIutl epitope of human mid-frontal gyrus brain tissue in immunohistochemistry.
• Form/Format: Lyophilized protein G purified in PBS pH7.4
• Sequence Length: 560

• Applicable Applications for anti-Vglut1 antibody: Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA (EIA), Western Blot (WB)
• Application Notes: If reconstituted in 100 muL: ICC/IHC: 1:5,000.
• Immunogen: Full Lenght VGIut1
• Preservative: None
• Preparation and Storage: Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.; To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).; For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Vglut1 antibody

Glutamate is the major excitatory neurotransmitter in the central nervous system of mammals. Vesicular glutamate transporters (VGIuts) are responsible for glutamate transport from the brain into native synaptic vesicles. The level of expression of the three known transporters in the adult brain determines the amount of glutamate that is loaded into vesicles and released.

References

1. Geissler M-Primary hippocampal neurons, which lack four crucial extracellular matrix molecules, display abnormalities of synaptic structure and function. 2. Allard S-Correlation of cognitive performance and morphological changes in neocortical pyramidal neurons in aging. 3. Seo S-Spatial learning-induced accumulation of agmatine and glutamate at hippocampal CA1 synaptic terminals. 4. Leon WC-A novel transgenic rat model with a full Alzheimer's-like amyloid pathology displays pre-plaque intracellular amyloid-beta-associated cognitive. 5. Ellegood J-Anatomical phenotyping in a mouse model of fragile X syndrome with magnetic resonance imaging. 6. Bell KF-ADAM-10 over-expression increases cortical synaptogenesis. 7. Bell KF-The amyloid pathology progresses in a neurotransmitter-specific manner.

NCBI and Uniprot Product Information

• NCBI GI #: 9945322
• NCBI GeneID: 57030
• NCBI Accession #: NP_064705.1
• NCBI GenBank Nucleotide #: NM_020309.4
• UniProt Accession #: Q9P2U7; Q6PCD0; B4DFR9; B4DG46
• Molecular Weight: 53,946 Da
• NCBI Official Full Name: vesicular glutamate transporter 1
• NCBI Official Synonym Full Names: solute carrier family 17 member 7
• NCBI Official Symbol: SLC17A7
• NCBI Official Synonym Symbols: BNPI; VGLUT1
• NCBI Protein Information: vesicular glutamate transporter 1
• UniProt Protein Name: Vesicular glutamate transporter 1
• UniProt Gene Name: SLC17A7
• UniProt Synonym Gene Names: BNPI; VGLUT1; VGluT1

NCBI Description

The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family. [provided by RefSeq, Jul 2008]

Uniprot Description

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.