Immunohistochemistry (IHC)
(Formalin-paraffin human tonsil stained with CD74 Ab (SPM523). Note membrane staining.)
Mouse anti-Human, Mouse CD74 Monoclonal Antibody | anti-CD74 antibody
CD74 (CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-Associated invariant chain, HLADR-gamma, HLADG, Ia antigen-Associated invariant chain, la-gamma, Major histocompatibility complex cla
Reactivity
Human, Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
CD74; Monoclonal Antibody; CD74 (CLIP; DHLAG; Gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-Associated invariant chain; HLADR-gamma; HLADG; Ia antigen-Associated invariant chain; la-gamma; Major histocompatibility complex cla; CLIP; Major histocompatibility complex class II invariant chain; MHC HLA-DR gamma chain; anti-CD74 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
SPM523
Specificity
Recognizes CD74. Species Crossreactivity: Human, Mouse
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Applicable Applications for anti-CD74 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 1-2ug/500ug protein lysate
FC/FACS: 0.5-1ug/million cells
IHC: 0.5-1ug/ml for 30 minutes at RT
IF: 1-2ug/ml
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
FC/FACS: 0.5-1ug/million cells
IHC: 0.5-1ug/ml for 30 minutes at RT
IF: 1-2ug/ml
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
Immunogen
SU-DHL-4 lymphoma cells. Cellular Localization: Cell surface and paranuclear globular
Conjugate
Biotin
Positive Control
Daudi Cells. Tonsil or lymph node.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-paraffin human tonsil stained with CD74 Ab (SPM523). Note membrane staining.)
Immunohistochemistry (IHC)
(Formalin-paraffin human tonsil stained with CD74 Ab (SPM523). Note membrane staining.)
Product Categories/Family for anti-CD74 antibody
References
1. Epstein AL et. al. J of Immunology 133: 1028-1036, 1984. 2. Marder RJ et. al. Lab Invest 52: 497-504, 1985.
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
3341
Protein Family
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Product Notes
The CD74 (Catalog #AAA6124038) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD74 (CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-Associated invariant chain, HLADR-gamma, HLADG, Ia antigen-Associated invariant chain, la-gamma, Major histocompatibility complex cla reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD74 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IP: 1-2ug/500ug protein lysate FC/FACS: 0.5-1ug/million cells IHC: 0.5-1ug/ml for 30 minutes at RT IF: 1-2ug/ml WB: 0.5-1ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD74 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD74, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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