Mouse UNC45a Monoclonal Antibody | anti-UNC45A antibody

UNC45a monoclonal antibody (AbS1)

Cat. Num. AAA565099

• Gene Names: UNC45A; SMAP1; SMAP-1; GCUNC45; UNC-45A; GC-UNC45; GCUNC-45; IRO039700
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Protein G affinity purified.
• Synonyms: UNC45a; Monoclonal Antibody; UNC45a monoclonal antibody (AbS1); anti-UNC45A antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: AbS1
• Specificity: Recognizes human, mouse and rat UNC45. Detects a band of ~98kDa by Western blot.
• Purity/Purification: Protein G affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 944

• Applicable Applications for anti-UNC45A antibody: Western Blot (WB)
• Application Notes: Western Blot (1:1,000, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~98kDa by Western blot.
• Immunogen: Recombinant human UNC45.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of UNC45: Lane 1: MW marker, Lane 2: HeLa, Lane 3: 3T3, Lane 4: PC-12.)

Related Product Information for anti-UNC45A antibody

GC UNC45 (general cell UNC45/unc45a) is an Hsp90 co-chaperone that has been shown to play a critical role in the chaperoning of myosin proteins as well as the progesterone receptor (PR). There are two known vertebrate isoforms of UNC45 that are functional orthologs of the C. elegans unc-45, an important protein in the development of organized body wall muscles. The vertebrate general cell form (GC UNC45/unc45a) is ubiquitously expressed, while the striated muscle-specific isoform (SM UNC45/unc45b) is found in cardiac and skeletal muscle. In vitro, GC UNC45 preferentially binds the Hsp90 beta isoform, and is specifically required for proper cellular distribution of Hsp90 beta but not Hsp90 alpha .

Product Categories/Family for anti-UNC45A antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 74761419
• NCBI GeneID: 55898
• UniProt Accession #: Q9H3U1; Q7L3Y6; Q9H3U8; Q9NSE8; Q9NSE9; A8K6F7
• Molecular Weight: 24,689 Da
• NCBI Official Full Name: Protein unc-45 homolog A
• NCBI Official Synonym Full Names: unc-45 myosin chaperone A
• NCBI Official Symbol: UNC45A
• NCBI Official Synonym Symbols: SMAP1; SMAP-1; GCUNC45; UNC-45A; GC-UNC45; GCUNC-45; IRO039700
• NCBI Protein Information: protein unc-45 homolog A
• UniProt Protein Name: Protein unc-45 homolog A
• Protein Family: Protein
• UniProt Gene Name: UNC45A
• UniProt Synonym Gene Names: SMAP1; Unc-45A; SMAP-1
• UniProt Entry Name: UN45A_HUMAN

NCBI Description

UNC45A plays a role in cell proliferation and myoblast fusion, binds progesterone receptor (PGR; MIM 607311) and HSP90 (HSPCA; MIM 140571), and acts as a regulator of the progesterone receptor chaperoning pathway (Price et al., 2002 [PubMed 12356907]; Chadli et al., 2006 [PubMed 16478993]).[supplied by OMIM, Mar 2008]

Uniprot Description

UNC45A: Acts as co-chaperone for HSP90. Prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. Positive factor in promoting PGR function in the cell. May be necessary for proper folding of myosin (Potential). Necessary for normal cell proliferation. Necessary for normal myotube formation and myosin accumulation during muscle cell development. May play a role in erythropoiesis in stroma cells in the spleen. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Cytoskeletal

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: nucleus; perinuclear region of cytoplasm

Molecular Function: Hsp90 protein binding; protein binding

Biological Process: cell differentiation; muscle development