Western Blot (WB)
(Western blot analysis of PKC theta expression in MCF7 (A), Jurkat (B) whole cell lysates.)
Rabbit anti-Human PKC theta Polyclonal Antibody | anti-PRKCQ antibody
Anti-PKC theta Antibody
Western Blot (WB)
(Western blot analysis of PKC theta expression in MCF7 (A), Jurkat (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PKC theta expression in MCF7 (A), Jurkat (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PKC theta staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PKC theta staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]
Uniprot Description
PKCT: an AGC kinase of the PKC family. A novel PKC: Ca2+ independent but still regulated by PS, DAG and phorbol esters. Contains 2 zinc-dependent phorbol-ester and DAG binding domains. Expressed in skeletal muscle and hematopoietic cells. May play a role in T cell receptor signaling and insulin resistance. Required for TCR-induced NF-kappaB activation in mature T lymphocytes.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Protein kinase, AGC; AGC group; PKC family; Delta subfamily
Chromosomal Location of Human Ortholog: 10p15
Cellular Component: cytosol; immunological synapse; intracellular; neuromuscular junction; nucleus; plasma membrane; sarcolemma
Molecular Function: ATP binding; metal ion binding; protein binding; protein kinase C activity; protein serine/threonine kinase activity; ubiquitin-protein ligase activity
Biological Process: activation of NF-kappaB transcription factor; aging; apoptosis; axon guidance; blood coagulation; cell structure disassembly during apoptosis; inflammatory response; innate immune response; membrane protein ectodomain proteolysis; negative regulation of insulin receptor signaling pathway; peptidyl-serine phosphorylation; phototransduction, visible light; platelet activation; positive regulation of filopodium formation; positive regulation of interleukin-17 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of NF-kappaB import into nucleus; positive regulation of protein secretion; positive regulation of stress fiber formation; positive regulation of T cell activation; positive regulation of T cell proliferation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; programmed cell death; protein ubiquitination; regulation of cell growth; regulation of G2/M transition of mitotic cell cycle; regulation of rhodopsin mediated signaling; regulation of transcription, DNA-dependent; regulation of vasoconstriction; respiratory burst; response to glucose stimulus; response to heat; response to hypoxia; response to insulin stimulus; response to organic cyclic substance; rhodopsin mediated signaling; T cell receptor signaling pathway; tissue regeneration
Research Articles on PRKCQ
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Product Notes
The PRKCQ prkcq (Catalog #AAA8243032) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PKC theta Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKC theta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the PRKCQ prkcq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC theta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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