Rabbit anti-Human UNC45A Polyclonal Antibody | anti-UNC45A antibody

UNC45A, NT (UNC45A, SMAP1, Protein unc-45 homolog A, GCUNC-45, Smooth muscle cell-associated protein 1) (APC)

Cat. Num. AAA6361455

• Gene Names: UNC45A; SMAP1; SMAP-1; GCUNC45; UNC-45A; GC-UNC45; GCUNC-45; IRO039700
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UNC45A; Polyclonal Antibody; NT (UNC45A; SMAP1; Protein unc-45 homolog A; GCUNC-45; Smooth muscle cell-associated protein 1) (APC); Smooth muscle cell-associated protein 1; anti-UNC45A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 944

• Applicable Applications for anti-UNC45A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UNC45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the N-terminal region of human UNC45A.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(UNC45A Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the UNC45A antibody detected the UNC45A protein (arrow).)

Related Product Information for anti-UNC45A antibody

UNC45A plays a role in cell proliferation and myoblast fusion, binds progesterone receptor (PGR; MIM 607311) and HSP90 (HSPCA; MIM 140571), and acts as a regulator of the progesterone receptor chaperoning pathway (Price et al., 2002 [PubMed 12356907]; Chadli et al., 2006 [PubMed 16478993]).

Product Categories/Family for anti-UNC45A antibody

Antibodies; Hormones; Steroids

References

Chadli, A., et al. J. Biol. Chem. 283(15):9509-9512(2008). Bazzaro, M., et al. Am. J. Pathol. 171(5):1640-1649(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 29725607
• NCBI GeneID: 55898
• NCBI Accession #: NP_061141.2; NP_001034764.1
• NCBI GenBank Nucleotide #: NP_061141.2
• UniProt Accession #: Q9H3U1
• NCBI Official Full Name: protein unc-45 homolog A isoform 2
• NCBI Official Synonym Full Names: unc-45 myosin chaperone A
• NCBI Official Symbol: UNC45A
• NCBI Official Synonym Symbols: SMAP1; SMAP-1; GCUNC45; UNC-45A; GC-UNC45; GCUNC-45; IRO039700
• NCBI Protein Information: protein unc-45 homolog A
• UniProt Protein Name: Protein unc-45 homolog A
• Protein Family: Protein
• UniProt Gene Name: UNC45A
• UniProt Synonym Gene Names: SMAP1; Unc-45A; SMAP-1
• UniProt Entry Name: UN45A_HUMAN

NCBI Description

This gene encodes a regulatory component of the progesterone receptor/heat shock protein 90 chaperoning complex, which functions in the assembly and folding of the progesterone receptor. The encoded protein is thought to be essential for normal cell proliferation, and for the accumulation of myosin during development of muscle cells. [provided by RefSeq, Sep 2018]

Uniprot Description

UNC45A: Acts as co-chaperone for HSP90. Prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. Positive factor in promoting PGR function in the cell. May be necessary for proper folding of myosin (Potential). Necessary for normal cell proliferation. Necessary for normal myotube formation and myosin accumulation during muscle cell development. May play a role in erythropoiesis in stroma cells in the spleen. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Cytoskeletal

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: nucleus; perinuclear region of cytoplasm

Molecular Function: Hsp90 protein binding; protein binding

Biological Process: cell differentiation; muscle development